ABSTRACT The objective of this study was to evaluate the serum protein pattern and selected acute phase proteins in dairy cows with chronic diarrhoea associated with seropositivity to Mycobacterium avium subsp. paratuberculosis (MAP). Forty-four dairy cows with chronic diarrhoea and MAP-seropositive and 19 clinically healthy MAP-seronegative cows were included in the study. The concentrations of total protein (TP), protein fractions and selected acute phase proteins – serum amyloid A (SAA), haptoglobin (Hp) and C-reactive protein (CRP) were measured in blood serum. In cows with diarrhoea the mean values of TP, albumin and the albumin/globulin ratio were significantly lower (P < 0.001), the relative concentrations of α1-, β1- and γ-globulins were significantly (P < 0.001) and α2- and β2-globulins were non-significantly higher. The electrophoretic pattern of serum proteins showed β-γ bridging in 32 from 44 diseased cows. The concentrations of SAA and Hp were non-significantly higher and CRP significantly lower (P < 0.001) in cows with diarrhoea. Presented results indicate a marked effect of chronic diarrhoea in MAP-seropositive cows on the protein metabolism suggesting possible diagnostic significance of some biomarkers from the protein profile in the evaluation of the severity of the disease and changes caused by this protein-losing enteropathy.

中文翻译：

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慢性腹泻对鸟分枝杆菌亚种的影响。副结核血清阳性奶牛的血清蛋白组分和选定的急性期蛋白

摘要 本研究的目的是评估与鸟分枝杆菌亚种血清阳性相关的慢性腹泻奶牛的血清蛋白模式和选定的急性期蛋白。副结核病 (MAP)。该研究包括 44 头患有慢性腹泻和 MAP 血清反应阳性的奶牛和 19 头临床健康的 MAP 血清反应阴性奶牛。在血清中测量总蛋白 (TP)、蛋白组分和选定的急性期蛋白——血清淀粉样蛋白 A (SAA)、结合珠蛋白 (Hp) 和 C 反应蛋白 (CRP) 的浓度。在腹泻奶牛中，TP、白蛋白和白蛋白/球蛋白比的平均值显着较低（P < 0.001），α1-、β1-和γ-球蛋白的相对浓度显着（P < 0.001）和α2-和β2-球蛋白非显着升高。血清蛋白的电泳图谱显示 44 头患病奶牛的 32 头出现 β-γ 桥连。腹泻奶牛的SAA和Hp浓度不显着升高，CRP显着降低（P < 0.001）。所呈现的结果表明，MAP 血清阳性奶牛的慢性腹泻对蛋白质代谢有显着影响，这表明蛋白质谱中的一些生物标志物在评估疾病严重程度和由这种蛋白质丢失性肠病引起的变化方面可能具有诊断意义。