ABSTRACT The present experiment investigated the effects of galacto-oligosaccharide (GOS) on growth performance, faecal microbiota, immune response and antioxidant capability in weaned piglets. Ninety 28-day-old weaned piglets were randomly allocated into 5 treatments with 6 replicate pens per treatment and 3 piglets per pen (n = 18). These five diets were formulated by adding 0, 500, 1000, 1500 and 2000 mg/kg GOS to the basal diet, respectively. The experiment was lasted for 28 days. Body weight and feed intake were determined. Faeces samples were collected to detect the amount of microbiota. Blood samples were used to determine antioxidative and immune parameters. The results indicated that GOS supplementation improved the growth performance, increased the number of Lactobacillus and Bifidobacteria and decreased the number of Escherichia coli in a linear or quadratic dose-dependent manner. Dietary GOS decreased serum concentration of pro-inflammatory cytokines in a quadratic dose-dependent manner, but increased anti-inflammatory cytokines in a linear or quadratic dose-dependent manner. Moreover, GOS supplementation promoted the activities of antioxidant enzyme in a linear or quadratic dose-dependent manner during the experiment. The levels of MDA quadratically decreased on d 28. These results suggested that GOS exhibited positive effects on growth performance, immune function and antioxidant capability in weaned piglets.

中文翻译：

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低聚半乳糖对断奶仔猪生长性能、粪便微生物群、免疫反应和抗氧化能力的影响

摘要 本实验研究了低聚半乳糖 (GOS) 对断奶仔猪生长性能、粪便微生物群、免疫反应和抗氧化能力的影响。90 头 28 日龄断奶仔猪随机分为 5 个处理，每个处理 6 个重复围栏，每个围栏 3 头仔猪（n = 18）。这五种日粮分别通过在基础日粮中添加 0、500、1000、1500 和 2000 mg/kg GOS 来配制。实验持续了28天。测定体重和采食量。收集粪便样本以检测微生物群的数量。血液样本用于确定抗氧化和免疫参数。结果表明，GOS 的补充提高了生长性能，以线性或二次剂量依赖性方式增加乳杆菌和双歧杆菌的数量并减少大肠杆菌的数量。膳食GOS以二次剂量依赖性方式降低促炎细胞因子的血清浓度，但以线性或二次剂量依赖性方式增加抗炎细胞因子。此外，在实验过程中，GOS 补充剂以线性或二次剂量依赖性方式促进抗氧化酶的活性。MDA 水平在第 28 天呈二次方下降。这些结果表明 GOS 对断奶仔猪的生长性能、免疫功能和抗氧化能力具有积极影响。但以线性或二次剂量依赖性方式增加抗炎细胞因子。此外，在实验过程中，GOS 补充剂以线性或二次剂量依赖性方式促进抗氧化酶的活性。MDA 水平在第 28 天呈二次方下降。这些结果表明 GOS 对断奶仔猪的生长性能、免疫功能和抗氧化能力具有积极影响。但以线性或二次剂量依赖性方式增加抗炎细胞因子。此外，在实验过程中，GOS 补充剂以线性或二次剂量依赖性方式促进抗氧化酶的活性。MDA 水平在第 28 天呈二次方下降。这些结果表明 GOS 对断奶仔猪的生长性能、免疫功能和抗氧化能力具有积极影响。