ABSTRACT Intestinal fatty-acid binding protein (I-FABP, also called FABP2) is involved in the uptake of dietary fatty acids in the intestine, and its binding specificity is only with long-chain fatty acid. In this study, sequences of 4 exons in pigeon FABP2 were successfully amplified. The encoded protein had a molecular weight of 15.13 kDa and the theoretical pI was 6.75. Bioinformatics analysis indicated that the protein was predicted to contain 10 potential phosphorylation sites and did not contain signal peptide structure. The evolutionary analysis suggested the amino acid sequence of pigeon FABP2 was closest to the mallard (Nippoia nippon) homologs. Meanwhile the expression of FABP2 mRNA in different ages and tissues we conducted. It showed that the expression of the FABP2 gene was the highest in the liver, followed by the small intestine and adipose tissue, while lowest in the breast muscle. Also, the expression level in the gizzard was gradually increased with aging. Our results may serve as reference to better studies of the functional characterization and structure of the FABP2 in pigeon.

中文翻译：

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家鸽（Columba livia）肠道型脂肪酸结合蛋白的生物信息学分析及组织特异性表达

摘要 肠道脂肪酸结合蛋白（I-FABP，也称为 FABP2）参与肠道对膳食脂肪酸的摄取，其结合特异性仅与长链脂肪酸结合。本研究成功扩增了鸽子FABP2的4个外显子序列。编码的蛋白质的分子量为 15.13 kDa，理论 pI 为 6.75。生物信息学分析表明，该蛋白质预计含有 10 个潜在的磷酸化位点，不含信号肽结构。进化分析表明鸽子FABP2的氨基酸序列与野鸭（Nippoia nippon）同源物最接近。同时我们进行了FABP2 mRNA在不同年龄和组织中的表达。表明 FABP2 基因在肝脏中的表达量最高，其次是小肠和脂肪组织，而在胸肌中最低。此外，随着年龄的增长，肌胃中的表达水平逐渐增加。我们的结果可以作为更好地研究鸽子中 FABP2 的功能特征和结构的参考。