Abstract This paper presents the preparation of stable fluorescence sensor slides for Hg2+ by covalent attachment of a fluorophore which can selectively chelate with the analyte. The fluorophore, dansyl chloride, was immobilized on the glass slide through an amide bond formation reaction with the amino group functionalized on the substrate using 3-aminopropyltriethoxysilane as the salinization agent. The dansyl chloride sensor slide was characterized by Fourier transform infrared (FTIR), ultraviolet-visible and fluorescence spectra. The size of sensor slide was precisely designed to fit in a common cuvette (10 × 10 mm). The ultraviolet-visible absorption and fluorescence response of the sensor to Hg2+ was directly investigated applying conventional spectrophotometers. The results showed that the sensor fluorescence may be linearly quenched by Hg2+ across the concentration range from 3.32 × 10−6 to 26.6 × 10−6 mol/L in 1:1 acetonitrile:water at pH 7.4. In addition, the sensor showed very high sensitivity to Hg2+ compared to other metal cations. Furthermore, the sensor slide was successfully applied for the determination of mercury ion in lake water with a recovery from 95.5 to 99.9%. This investigation has demonstrated that the proposed approach may be used to develop a stable, accurate, simple, and selective fluorescence sensor for Hg2+.

中文翻译：

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使用丹磺酰氯固定载玻片通过荧光法测定水样中的汞 (II)

摘要 本文介绍了通过共价连接荧光团来制备稳定的 Hg2+ 荧光传感器载玻片，荧光团可以选择性地与分析物螯合。荧光团丹磺酰氯通过酰胺键形成反应固定在载玻片上，使用 3-氨基丙基三乙氧基硅烷作为盐化剂，在基板上官能化氨基。丹磺酰氯传感器载玻片的特征在于傅里叶变换红外 (FTIR)、紫外-可见光和荧光光谱。传感器载玻片的尺寸经过精确设计以适合普通比色皿 (10 × 10 mm)。使用常规分光光度计直接研究传感器对 Hg2+ 的紫外-可见吸收和荧光响应。结果表明，在 3.32 × 10−6 到 26.6 × 10−6 mol/L 的 1:1 乙腈:水中 pH 7.4 的浓度范围内，Hg2+ 可以线性淬灭传感器荧光。此外，与其他金属阳离子相比，该传感器对 Hg2+ 表现出非常高的灵敏度。此外，传感器载玻片成功应用于湖水中汞离子的测定，回收率从 95.5% 到 99.9%。该研究表明，所提出的方法可用于开发稳定、准确、简单和选择性的 Hg2+ 荧光传感器。传感器载玻片成功应用于湖水中汞离子的测定，回收率为95.5%至99.9%。该研究表明，所提出的方法可用于开发稳定、准确、简单和选择性的 Hg2+ 荧光传感器。传感器载玻片成功应用于湖水中汞离子的测定，回收率为95.5%至99.9%。该研究表明，所提出的方法可用于开发稳定、准确、简单和选择性的 Hg2+ 荧光传感器。