Abstract Raman spectroscopy is a rapid technique that allows the simultaneous determination of several components of urine to assess the metabolic status. In this study, the urine composition was evaluated in volleyball and soccer athletes using Raman spectroscopy to identify biomarkers of sports performance. Urine samples were collected from 20 athletes before and after exercise to be analyzed with a Raman microscope with excitation at 532 nm and a laser power of 67 mW. The analyses were compared with a group of 10 non-sports subjects. Raman analyses show peaks associated with the presence of urea, uric acid, creatinine, and ketone compounds with different intensity before and after exercise. The concentrations of urea, creatinine, and ketones were determined by commercial kits and were compared with the Raman analyses. The excretion of urea in urine increased after exercise in most volleyball athletes, while in some soccer players it decreased. The creatinine concentration increased significantly with exercise. The presence of ketones was observed in all of the spectra after exercise. The excretion of these metabolites is the result of the intensity and physical activity performed. Through this technique, we can evaluate athletes under training to establish their metabolic conditions and make nutritional adjustments to improve their athletic performance.

中文翻译：

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拉曼光谱技术在排球和足球运动员代谢状态的鉴别诊断中的应用

摘要 拉曼光谱是一种快速技术，可以同时测定尿液的几种成分以评估代谢状态。在这项研究中，使用拉曼光谱评估排球和足球运动员的尿液成分，以确定运动表现的生物标志物。从 20 名运动员运动前后收集尿液样本，用拉曼显微镜进行分析，激发波长为 532 nm，激光功率为 67 mW。这些分析与一组 10 名非运动受试者进行了比较。拉曼分析显示了与运动前后不同强度的尿素、尿酸、肌酐和酮化合物的存在相关的峰值。尿素、肌酐和酮的浓度由商业试剂盒测定，并与拉曼分析进行比较。大多数排球运动员在运动后尿液中尿素的排泄量增加，而一些足球运动员则减少。肌酐浓度随着运动而显着增加。在运动后的所有光谱中都观察到酮的存在。这些代谢物的排泄是强度和体力活动的结果。通过这种技术，我们可以评估训练中的运动员，以建立他们的代谢状况并进行营养调整以提高他们的运动表现。这些代谢物的排泄是强度和体力活动的结果。通过这种技术，我们可以评估训练中的运动员，以建立他们的代谢状况并进行营养调整以提高他们的运动表现。这些代谢物的排泄是强度和体力活动的结果。通过这种技术，我们可以评估训练中的运动员，以建立他们的代谢状况并进行营养调整以提高他们的运动表现。