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SRAP markers for genetic diversity assessment of lavender (Lavandula angustifolia mill.) varieties and breeding lines
Biotechnology & Biotechnological Equipment ( IF 1.5 ) Pub Date : 2020-01-01 , DOI: 10.1080/13102818.2020.1742788
Tzvetelina Zagorcheva 1 , Stanko Stanev 2 , Krasimir Rusanov 1 , Ivan Atanassov 1
Affiliation  

Abstract Lavender (Lavandula angustifolia Mill.) is an essential oil-bearing and medicinal plant of great economic and social importance to Bulgaria, which is the world largest producer of lavender oil nowadays. Currently, the industrial cultivation of lavender in Bulgaria employs up to seven varieties established during the last century. Despite the growing industrial lavender cultivation, few molecular markers have been applied for characterization of lavender genetic resources and breeding. The present study employed sequence-related amplified polymorphism (SRAP) markers to characterize the genetic resources and genetic diversity within and between two groups of ten Bulgarian and five foreign lavender varieties and breeding lines. The data generated following analysis with 51 SRAP primer pairs showed amplification of a high number (4697) and ratio (77.2%) of polymorphic SRAP fragments resulting in relatively high value of the polymorphism information content, with an average of 0.27 ± 0.03 for the used primer pairs. Analysis of molecular variance of the SRAP data further revealed high level of genetic diversity (96.6% of the total variations) within the analyzed groups of Bulgarian and foreign varieties and rather low diversity (3.4% of total variations) between the two groups. The possibilities for further applications of SRAP markers in lavender breeding and cultivation are discussed.

中文翻译:

用于薰衣草 (Lavandula angustifolia mill.) 品种和育种系遗传多样性评估的 SRAP 标记

摘要 薰衣草(Lavandula angustifolia Mill.)是当今世界上最大的薰衣草油生产国保加利亚,是一种具有重要经济和社会意义的精油和药用植物。目前,保加利亚的薰衣草工业种植采用了上个世纪建立的多达七个品种。尽管工业薰衣草种植不断增长,但很少有分子标记用于薰衣草遗传资源和育种的表征。本研究采用序列相关的扩增多态性 (SRAP) 标记来表征两组内部和之间的遗传资源和遗传多样性,包括十个保加利亚和五个外国薰衣草品种和育种系。使用 51 个 SRAP 引物对分析后生成的数据显示,多态性 SRAP 片段的数量(4697)和比例(77.2%)扩增,导致多态性信息含量的值相对较高,所使用的平均为 0.27 ± 0.03引物对。SRAP 数据的分子方差分析进一步揭示了保加利亚和外国品种分析组内的高水平遗传多样性(总变异的 96.6%)和两组之间的低多样性(总变异的 3.4%)。讨论了 SRAP 标记在薰衣草育种和栽培中进一步应用的可能性。SRAP 数据的分子方差分析进一步揭示了保加利亚和外国品种分析组内的高水平遗传多样性(总变异的 96.6%)和两组之间的低多样性(总变异的 3.4%)。讨论了 SRAP 标记在薰衣草育种和栽培中进一步应用的可能性。SRAP 数据的分子方差分析进一步揭示了保加利亚和外国品种分析组内的高水平遗传多样性(总变异的 96.6%),而两组之间的多样性较低(总变异的 3.4%)。讨论了 SRAP 标记在薰衣草育种和栽培中进一步应用的可能性。
更新日期:2020-01-01
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