Abstract This study investigated the expression of inflammatory factors in tumour tissues, monocytes and blood of non-small cell lung cancer (NSCLC) patients taking tetrandrine (Tet) during low-dose radiotherapy. Sixty NSCLC patients underwent surgical resection of lung cancer after low-dose radiotherapy. Thirty patients did not take Tet (control group) and the other thirty patients took Tet during radiotherapy (experimental group). Lung cancer tissues were collected from patients, and tumour-adjacent tissues were collected as controls. Peripheral blood was collected on the morning of radiotherapy for mononuclear cell separation. Quantitative real-time polymerase chain reaction was used to determine mRNA expression, and Western blotting was used to measure protein expression. Enzyme-linked immunosorbent assay was used to determine the inflammatory factor contents in liquid samples. Cell proliferation was determined via MTT assay at 24, 48 and 72 h after transfection in A549 cells. Tet reduced COX-2 mRNA in serum and blood monocytes and decreased COX-2 protein expression in monocytes from patients with radiotherapy. It decreased the release of inflammatory factors to blood after radiotherapy. Combined treatment with low-dose radiotherapy and Tet tablets reduced the expression of Ki-67 protein in tumour tissues, and decreased the release of inflammatory factors from NSCLC tissues after radiotherapy. Combined treatment with low-dose radiotherapy and Tet tablets reduced the proliferation of cancer cells possibly through reducing inflammatory factors released by tumour tissues. The study demonstrates that Tet administration during low-dose radiotherapy reduces the release of inflammatory factors by NSCLC tissues, and decreases tumour proliferation. Tet plays an auxiliary role in NSCLC radiotherapy.

中文翻译：

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粉防己碱在非小细胞肺癌低剂量放疗过程中可减轻炎症并延缓肺癌的生长

摘要 本研究调查了非小细胞肺癌（NSCLC）患者在低剂量放疗期间服用粉防己碱（Tet）的肿瘤组织、单核细胞和血液中炎症因子的表达情况。60 例非小细胞肺癌患者在低剂量放疗后接受了肺癌手术切除。30 名患者未服用 Tet（对照组），另外 30 名患者在放疗期间服用 Tet（实验组）。从患者身上收集肺癌组织，并收集肿瘤邻近组织作为对照。放疗当日早晨采集外周血进行单核细胞分离。定量实时聚合酶链反应用于测定 mRNA 表达，蛋白质印迹用于测量蛋白质表达。采用酶联免疫吸附法测定液体样品中炎症因子的含量。在 A549 细胞中转染后 24、48 和 72 小时，通过 MTT 测定确定细胞增殖。Tet 降低了血清和血液单核细胞中的 COX-2 mRNA，并降低了放疗患者单核细胞中 COX-2 蛋白的表达。减少放疗后炎症因子释放到血液中。小剂量放疗和Tet片联合治疗降低了肿瘤组织中Ki-67蛋白的表达，减少了放疗后NSCLC组织炎症因子的释放。低剂量放疗和Tet片剂联合治疗可能通过减少肿瘤组织释放的炎症因子来减少癌细胞的增殖。该研究表明，在低剂量放疗期间使用 Tet 可减少 NSCLC 组织释放炎症因子，并减少肿瘤增殖。Tet在NSCLC放疗中起辅助作用。