Abstract The potential of the indigenously isolated biosurfactant producing organism, Paenibacillus sp. D9 to utilize waste frying oils (canola, sunflower, castor, and coconut) were investigated as alternate cheap substrates for synthesis of biosurfactant. This work aimed to study optimum parameters, functional properties, and applicability for bioremediation. Initial biosurfactant yield showed 2.11 g/L and 33.7 mN/m, a representative of low production. There was an improvement on maximum reduction in surface tension and biosurfactant yield of 31.2 mN/m and 5.31 g/L, respectively as the two quadratic models were adequate with regards to biosurfactant yield (R2 = 0.9937) and surface tension (R2 = 0.9862). The proficiency of biosurfactant when contrasted to a chemical surfactant bring about the degradation of 73.2% motor oil, 71.8% diesel (shaking condition), 63.3% motor oil, and 59.3% diesel (static condition) in a solid environment. In contrast, using an aqueous environment, there was bio-removal of 77.6% motor oil, 74.3% diesel (shaking condition), 62.2% motor oil, and 57.4% diesel (static condition) respectively. The response surface methodology tool was suitable in identifying the optimum conditions on low-cost substrates and the biosurfactant is capable for bioremediation of diesel and motor and from contaminated environment.

中文翻译：

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通过优化类芽孢杆菌产生的生物表面活性剂对污染柴油和机油进行生物修复。D9 废菜籽油

摘要本地分离的生物表面活性剂生产生物类芽孢杆菌的潜力。研究了利用废弃煎炸油（菜籽油、向日葵、蓖麻和椰子）的 D9 作为合成生物表面活性剂的替代廉价底物。这项工作旨在研究生物修复的最佳参数、功能特性和适用性。初始生物表面活性剂产量显示为 2.11 g/L 和 33.7 mN/m，代表低产量。由于两个二次模型在生物表面活性剂产量 (R2 = 0.9937) 和表面张力 (R2 = 0.9862) 方面足够，因此表面张力和生物表面活性剂产量的最大降低分别提高了 31.2 mN/m 和 5.31 g/L . 与化学表面活性剂相比，生物表面活性剂的熟练程度会导致 73.2% 的机油降解，71。在固体环境中，8% 柴油（振动条件）、63.3% 机油和 59.3% 柴油（静态条件）。相比之下，使用水性环境，分别对 77.6% 的机油、74.3% 的柴油（振动条件）、62.2% 的机油和 57.4% 的柴油（静态条件）进行了生物去除。响应面方法工具适用于确定低成本基材的最佳条件，生物表面活性剂能够对柴油和发动机以及污染环境进行生物修复。