Abstract

The ability to accumulate nickel (Ni) was compared in hyperaccumulator Noccaea сaerulescens F.K. Mey and excluder Thlaspi arvense L. after a short-term (1, 2, or 3 days) and long-term (8 weeks) exposure. T. arvense and four accessions of N. сaerulescens (La Calamine (LC), Saint Félix de Palliéres (SF), Monte Prinzera (MP), and Lellingen (LE)) were grown on a half-strength Hoagland`s solution in the presence of 25 µM Ni(NO₃)₂ (N. сaerulescens and T. arvense) and 250 µM Ni(NO₃)₂ (N. сaerulescens; T. arvense for only 1–3 days). Metal content in the roots and shoots was determined by atomic absorption spectroscopy. The Ni content per unit mass in the roots and shoots of N. сaerulescens in most cases did not differ significantly after the short-term incubation. At 25 µM Ni in the nutrient solution, its content in the roots of LC plants after 2–3 days of incubation was lower than in T. arvense, whereas Ni content in the shoots of these plants was similar. In the plants of other accessions of N. сaerulescens, Ni content in the roots and shoots in most cases was higher than in T. arvense. At 250 µM Ni, the differences in metal content in the roots were insignificant, and its content in the shoots in all the accessions of the hyperaccumulator was much higher than in the excluder. The Ni translocation factor was higher in N. сaerulescens than in T. arvense and exceeded unity only in the plants of MP accession. After the long-term exposure, the Ni translocation factor was higher than 1 in plants of all accessions of N. сaerulescens and decreased in the following order: MP ≈ LC > LE ≥ SF; in T. arvense, it did not exceed 0.3. Upon both long-term and short-term exposure, the ability to accumulate Ni by N. сaerulescens plants of different accessions generally increased in the following order: LC < SF < LE < MP. However, minor changes were observed depending on the duration of exposure and Ni concentration in the medium. Thus, considerable differences in the ability to accumulate Ni among the plants of different accessions of hyperaccumulator N. сaerulescens became apparent as early as during the first days of exposure to Ni and hardly depended on the duration of incubation or metal concentration in the medium. The obtained data confirm the assumption about a constitutive or genetically predetermined ability of plants of different N. сaerulescens accessions to accumulate Ni.

中文翻译：

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短期和长期暴露后，引物Thlaspi arvense和Hyperaccumer caerulescens积累镍

摘要

在短期（1、2、3天）和长期（8周）暴露后，在超级蓄积夜蛾夜蛾FK Mey和排卵菌Thlaspi arvense L.中比较了镍的累积能力。T. arvense和N.сaerulescens（La Calamine（LC），SaintFélixdePalliéres（SF），Monte Prinzera（MP）和Lellingen（LE））的四个种在半强度Hoagland的溶液中生长存在25 µM Ni（NO ₃）₂（N.сaerulescens和T. arvense）和250 µM Ni（NO ₃）₂（N.сaerulescens; T. arvense仅1-3天）。根和芽中的金属含量通过原子吸收光谱法测定。短期孵育后，大多数情况下，芥蓝的根和茎中每单位质量的镍含量没有显着差异。营养液中的镍含量为25 µM时，培养2–3天后，其在LC植物根部的含量要低于白僵菌（T. arvense），而这些植物的芽中的镍含量却相似。在其他种的芥蓝猪笼草的植物中，大多数情况下，其根和茎中的镍含量都高于欧a草。。在250 µM Ni下，根系中金属含量的差异不明显，并且在超富集所有种质的枝条中其金属含量远高于排斥株。镍易位因素是较高的N.сaerulescens比兔足三叶草，只有在MP加入的植物超过统一。长期暴露后，所有芥菜种的植株中Ni的转运因子均高于1，并按以下顺序降低：MP≈LC> LE≥SF；在T. arvense中，不超过0.3。在长期和短期暴露下，芥蓝油菌积累镍的能力不同种质的植物通常按以下顺序增加：LC <SF <LE <MP。但是，根据暴露的持续时间和培养基中的Ni浓度，观察到微小的变化。因此，早在暴露于Ni的第一天期间，不同累积量的超级蓄积猪笼草的不同植物之间在积累Ni的能力上就存在明显差异，并且几乎不依赖于培养的持续时间或培养基中的金属浓度。所获得的数据证实了关于不同芥菜种的植物积累Ni的组成或遗传预定能力的假设。