The field efficacy of liquid formulations of Pseudomonas fluorescens, Purpureocillium lilacinum and Trichoderma viride was investigated against natural populations of potato cyst nematodes (PCNs) Globodera rostochiensis and Globodera pallida. Two field experiments were conducted using these biocontrol agents applied as seed treatment (ST) at 1 L t⁻¹ of seed and soil drenching (SD) at 5 L ha⁻¹. Their effect was compared with carbofuran 3 G at 1 kg a.i. ha⁻¹. Results showed that all the tested biocontrol agents were capable to reduce PCN egg density or multiplication rate, root penetration and egg numbers cyst⁻¹ at various levels. The biocontrol efficacy also varied according to their delivery method. P. fluorescens-ST was found causing the highest reduction of egg density (83.1–86.5%), multiplication rate (83.1–86.5%), root penetration (67.1–68.1%) and egg numbers cyst⁻¹ (35.4–36.0%). Similar efficacy was observed in P. lilacinum-SD that reduced egg density by 80.9–85.0%, multiplication rate by 80.7–84.3%, root penetration by 62.0–64.4% and egg numbers cyst⁻¹ by 44.3–49.3%. The potato plants from P. fluorescens-ST and P. lilacinum-SD plots were 31.1–49.5% taller than untreated plants which also resulted in 27.2–32.0% higher tuber yield in P. fluorescens-ST and 25.9–30.2% in P. lilacinum-SD plots. The root colonisation of introduced P. fluorescens was 8.6–13.1 times higher in ST than SD. The trend was vice versa in P. lilacinum that colonised 3.1–3.6 times better in SD method than as ST. Their efficacy was comparable to carbofuran application. It is concluded that P. fluorescens-ST at 1 L t⁻¹ of seed and P. lilacinum-SD at 5 L ha⁻¹ are highly effective for management of PCN under naturally infested field conditions.

的液体制剂领域功效荧光假单胞菌，Purpureocillium lilacinum和绿色木霉进行了研究针对马铃薯胞囊线虫（PCN类）的自然种群马铃薯球胞囊线虫和马铃薯金淡紫。使用这些生物防治剂进行了两个田间试验，这些生物防治剂在1 L t ^-1的种子施肥（ST）和5 L ha ^-1的土壤淋湿（SD）。将其效果与1 kg ai ha ^-1的呋喃丹3 G进行了比较。结果表明，所有测试的生物防治剂均具有降低PCN卵密度或繁殖率，根部穿透力和卵数囊肿^{-1的能力。}在各个层次上。生物防治功效还根据其递送方法而变化。发现荧光假单胞菌引起的卵密度降低最高（83.1–86.5％），繁殖率（83.1–86.5％），根渗透（67.1–68.1％）和卵数囊肿^-1（35.4–36.0％） 。在丁香假单胞菌-SD中观察到了类似的功效，它使卵密度降低了80.9–85.0％，繁殖率降低了80.7–84.3％，根渗透率降低了62.0–64.4％，卵囊数^-1降低了44.3–49.3％。来自荧光假单胞菌-ST和淡紫色假单胞菌-SD地块的马铃薯植株比未处理的植株高31.1–49.5％，这也导致荧光假单胞菌的块茎产量提高了27.2–32.0％-ST和紫丁香-SD图中的25.9–30.2％。引入的荧光假单胞菌的根定殖在ST中比SD高8.6-13.1倍。反之亦然，紫丁香假单胞菌在定植方法中的定殖率比普通定殖菌高3.1-3.6倍。它们的功效与呋喃丹的使用相当。结论是，在自然侵染的田间条件下，种子的1 L t ^-1处的荧光假单胞菌-ST和5 L ha ^-1处的淡紫色假单胞菌-SD对PCN的管理非常有效。