Abstract

Lysine succinylation of proteins has potential impacts on protein structure and function, which occurs on post-translation level. However, the information about the succinylation of proteins in tea plants is limited. In the present study, the significant signal of succinylation in tea plants was found by western blot. Subsequently, we performed a qualitative analysis to globally identify the lysine succinylation of proteins using high accuracy nano LC-MS/MS combined with affinity purification. As a result, a total of 142 lysine succinylation sites were identified on 86 proteins in tea leaves. The identified succinylated proteins were involved in various biological processes and a large proportion of the succinylation sites were presented on proteins in the primary metabolism, including glyoxylate and dicarboxylate metabolism, TCA cycle and glycine, serine and threonine metabolism. Moreover, 10 new succinylation sites were detected on histones in tea leaves. The results suggest that succinylated proteins in tea plants might play critical regulatory roles in biological processes, especially in the primary metabolism. This study not only comprehensively analyzed the lysine succinylome in tea plants, but also provided valuable information for further investigating the functions of lysine succinylation in tea plants.

中文翻译：

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蛋白质的蛋白质组全赖氨酸琥珀酰化分析表明其参与初级代谢

摘要

蛋白质的赖氨酸琥珀酰化对蛋白质的结构和功能有潜在的影响，发生在翻译后水平。但是，有关茶树中蛋白质琥珀酰化的信息是有限的。在本研究中，通过蛋白质印迹法发现了茶树中琥珀酰化的重要信号。随后，我们进行了定性分析，以使用高精度的纳米LC-MS / MS与亲和纯化相结合，全面鉴定蛋白质的赖氨酸琥珀酰化。结果，在茶叶中的86种蛋白质上鉴定出总共142个赖氨酸琥珀酰化位点。鉴定出的琥珀酰化蛋白参与了各种生物学过程，并且大部分的琥珀酰化位点出现在初级代谢的蛋白上，包括乙醛酸和二羧酸酯代谢，TCA循环和甘氨酸，丝氨酸和苏氨酸的代谢。此外，在茶叶的组蛋白上检测到10个新的琥珀酰化位点。结果表明，茶树中的琥珀酰化蛋白可能在生物过程中，尤其是在初级代谢中起着关键的调节作用。这项研究不仅全面分析了茶树中的赖氨酸琥珀酰化酶，而且为进一步研究茶树中赖氨酸的琥珀酰化作用提供了有价值的信息。