MADS-box genes interact with TB1 to regulate plant organ morphogenesis. In rice, OsMADS57 interacts with OsTB1 to control OsD14 transcription. In this study, we aimed to determine the relationships among these genes in barley. We identified a natural mutant of HvTB1 (tb1) formed by a C→A transition at position 230, which resulted in a premature stop codon. We cloned the HvMADS57 and HvD14 genes and studied their expression in the tb1 mutant. The results showed that HvMADS57 is a MIKCc-type MADS-box gene, and the expression levels of both HvMADS57 and HvD14 were significantly reduced in the tb1 mutant when compared to those in the wild-type gene. These results indicate that, HvMADS57 regulates plant growth and development by interacting with HvTB1 to suppress the transcription of HvD14 in barley which is similar to the relationships among the orthologs of these genes in rice.

中文翻译：

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HvMADS57 和 HvD14 在大麦 tb1 突变体中的鉴定和表达谱

MADS-box 基因与 TB1 相互作用以调节植物器官形态发生。在水稻中，OsMADS57 与 OsTB1 相互作用以控制 OsD14 转录。在这项研究中，我们旨在确定大麦中这些基因之间的关系。我们鉴定了 HvTB1 (tb1) 的天然突变体，该突变体由位置 230 处的 C→A 转换形成，这导致过早的终止密码子。我们克隆了 HvMADS57 和 HvD14 基因并研究了它们在 tb1 突变体中的表达。结果表明，HvMADS57 是一个 MIKCc 型 MADS-box 基因，与野生型基因相比，tb1 突变体中 HvMADS57 和 HvD14 的表达水平显着降低。这些结果表明，