In decapods, dopamine, octopamine, serotonin, and histamine function as locally released/hormonally delivered modulators of physiology/behavior. Although the functional roles played by amines in decapods have been examined extensively, little is known about the identity/diversity of their amine receptors. Recently, a Homarus americanus mixed nervous system transcriptome was used to identify putative neuronal amine receptors in this species. While many receptors were identified, some were fragmentary, and no evidence of splice/other variants was found. Here, the previously predicted proteins were used to search brain- and eyestalk ganglia-specific transcriptomes to assess/compare amine receptor complements in these portions of the lobster nervous system. All previously identified receptors were reidentified from the brain and/or eyestalk ganglia transcriptomes, i.e., dopamine alpha-1, beta-1, and alpha-2 (Homam-DAα2R) receptors, octopamine alpha (Homam-OctαR), beta-1, beta-2, beta-3, beta-4, and octopamine–tyramine (Homam-OTR-I) receptors, serotonin type-1A, type-1B (Homam-5HTR1B), type-2B, and type-7 receptors; and histamine type-1 (Homam-HA1R), type-2, type-3, and type-4 receptors. For many previously partial proteins, full-length receptors were deduced from brain and/or eyestalk ganglia transcripts, i.e., Homam-DAα2R, Homam-OctαR, Homam-OTR-I, and Homam-5HTR1B. In addition, novel dopamine/ecdysteroid, octopamine alpha-2, and OTR receptors were discovered, the latter, Homam-OTR-II, being a putative paralog of Homam-OTR-I. Finally, evidence for splice/other variants was found for many receptors, including evidence for some being assembly-specific, e.g., a brain-specific Homam-OTR-I variant and an eyestalk ganglia-specific Homam-HA1R variant. To increase confidence in the transcriptome-derived sequences, a subset of receptors was cloned using RT-PCR. These data complement/augment those reported previously, providing a more complete picture of amine receptor complement/diversity in the lobster nervous system.

在十足目动物中，多巴胺、章鱼胺、血清素和组胺起到局部释放/激素传递的生理/行为调节剂的作用。尽管胺在十足目中发挥的功能作用已被广泛研究，但对其胺受体的身份/多样性知之甚少。最近，美洲白鹤混合神经系统转录组被用来鉴定该物种中假定的神经元胺受体。虽然鉴定了许多受体，但有些受体是片段的，并且没有发现剪接/其他变体的证据。在这里，之前预测的蛋白质被用来搜索大脑和眼柄神经节特异性转录组，以评估/比较龙虾神经系统这些部分的胺受体补体。所有先前鉴定的受体均从大脑和/或眼柄神经节转录组中重新鉴定，即多巴胺 α-1、β-1 和 α-2 (Homam-DAα2R) 受体、章鱼胺 α (Homam-OctαR)、β-1、 beta-2、beta-3、beta-4 和章鱼胺-酪胺 (Homam-OTR-I) 受体，血清素 1A 型、1B 型 (Homam-5HTR1B)、2B 型和 7 型受体；和组胺 1 型 (Homam-HA1R)、2 型、3 型和 4 型受体。对于许多先前的部分蛋白，全长受体是从大脑和/或眼柄神经节转录物中推导出来的，即Homam-DAα2R、Homam-OctαR、Homam-OTR-I和Homam-5HTR1B。此外，还发现了新的多巴胺/蜕皮类固醇、章鱼胺α-2和OTR受体，后者Homam-OTR-II，是Homam-OTR-I的推定旁系同源物。最后，发现了许多受体存在剪接/其他变体的证据，包括某些装配特异性的证据，例如脑特异性 Homam-OTR-I 变体和眼柄神经节特异性 Homam-HA1R 变体。为了增加转录组衍生序列的可信度，使用 RT-PCR 克隆了受体的子集。这些数据补充/增强了之前报道的数据，提供了龙虾神经系统中胺受体补充/多样性的更完整的图景。