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Pathogenicity of Saudi Arabian fungal isolates against egg and larval stages of Rhynchophorus ferrugineus under laboratory conditions
International Journal of Tropical Insect Science ( IF 1.2 ) Pub Date : 2020-04-22 , DOI: 10.1007/s42690-020-00141-8
Lamya Ahmed Al-Keridis , Nevien M. Gaber , Abdulrahman Saad Aldawood

The pathogenicity of various local entomopathogenic fungal isolates from the kingdom of Saudi Arabia has been tested in the laboratory against the red palm weevil, Rhynchophorus ferrugineus Olivier (Coleoptera: Curculionidae). Fungi were coded, isolated, and purified. Beauveria bassiana (Balsamo-Crivelli) Vuillemin, coded as: BbSA-1 PDA-15 and Metarhizium anisopliae (Metsch.) Sorokin, coded as: MaSA-1 PDA-15, isolates demonstrated significant potential against R. ferrugineus larvae. A 1 × 1010 conidia/mL concentration of positive fungal isolates along with natural B. bassiana was used against different stages of R. ferrugineus. Rhynchophorus ferrugineus larvae were supplied with synthetic diets that contain preservatives and non-preservatives. The fungal isolates BbSA-1 PDA-15 and MaSA-1 PDA-15 affected the egg hatchability and caused significant larval mortality of red palm weevil. It was shown that MaSA-1 PDA-15 is one of the most pathogenic fungi against R. ferrugineus larvae followed by BbSA-1 PDA-15 and Natural B. bassiana isolates. Therefore, these local fungal isolates have the potential to be used against R. ferrugineus. Further development in the research could facilitate the farmers to save their valuable palm trees from the notorious pest.



中文翻译:

沙特阿拉伯真菌分离株在实验室条件下对蓝藻的卵和幼虫期的致病性

来自沙特阿拉伯王国的各种地方病原性真菌分离株的致病性已在实验室中针对红色棕榈象鼻虫Rhynchophorus ferrugineus Olivier(鞘翅目:Curculionidae)进行了测试。对真菌进行编码,分离和纯化。球孢白僵菌(Balsamo的-CRIVELLI)Vuillemin,编码为:BBSA-1 PDA-15和绿僵菌(Metsch)索罗金,编码为:MASA-1 PDA-15,菌株表现出对显著潜在红棕象甲幼虫。以1×10 10分生孢子/ mL浓度的阳性真菌分离物与天然球孢白僵菌一起用于对抗不同阶段的R. ferrugineus含营养防腐剂和非防腐剂的合成日粮中提供了铁蓝藻幼虫。真菌分离物BbSA-1 PDA-15和MaSA-1 PDA-15影响了卵的孵化率并导致了红掌象鼻虫的明显幼虫死亡率。结果表明,MaSA-1 PDA-15是针对R. ferrugineus幼虫的最具致病性的真菌之一,其次是BbSA-1 PDA-15和天然B. bassiana分离株。因此,这些局部真菌分离物具有抗铁锈菌的潜力。该研究的进一步发展可以促进农民从臭名昭著的害虫中拯救其宝贵的棕榈树。

更新日期:2020-04-22
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