ITS1 and ITS2 of ribosomal DNA (rDNA) of six subfamilies i.e. Epilachaninae, Chilocorinae, Coccinellinae, Coccidulinae, Scymininae Ortaliinae and Sticholotidinae were examined, aligned and conserved motifs in folding patterns of stable consensus secondary structures were assessed. RNAalifold and RNA fold algorithms applied by RNAz program to compute consensus secondary structures and MFE efficiently and derive a number of statistical scores i.e. SCI, Z-score and MPI. Assembled subsets in ITS1 (three sets) and ITS2 (ten sets) were significantly conserved. In this study, analysis of these consensus secondary structures shows that a motif sequence is remain conserved in all the species. Helices in subsets of ITS1 showed compensatory base involvement to maintain the stability and conservation of secondary structures during natural selection. Secondary structures of ITS2 found more conserved than ITS1. MP, BI and ML methods used to establish the evolutionary relationship, compared the topology significance on the basis of SCI and MPI in defined sets. Coccinellinae, Epilachaninae and Scymninae (all strains of species) were monophyletic whereas Coccidulinae, and Chilocorinae had inadequate sequence information. Of Ortaliinae and Sticholotidinae, yet no ITS sequences have been submitted. Thus, analysis of the structural folding patterns of ITS regions provided further prime information of species relatedness and inter-intra-specific variations.

中文翻译：

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瓢虫科 ITS 二级结构保守性和协变的广泛检查和比较分析

对六个亚科（即 Epilachaninae、Chilocorinae、Coccinellinae、Coccidulinae、Scymininae Ortaliinae 和 Sticholotidinae）的核糖体 DNA (rDNA) 的 ITS1 和 ITS2 进行了检查，评估了稳定共有二级结构的折叠模式中的对齐和保守基序。RNAifold 和 RNA 折叠算法由 RNAz 程序应用，以有效计算共有二级结构和 MFE，并得出许多统计分数，即 SCI、Z-score 和 MPI。ITS1（三组）和 ITS2（十组）中的组装子集显着保守。在这项研究中，对这些共有二级结构的分析表明，一个基序序列在所有物种中都保持保守。ITS1 子集中的螺旋显示出补偿性碱基参与，以在自然选择过程中维持二级结构的稳定性和保守性。发现 ITS2 的二级结构比 ITS1 更保守。用于建立进化关系的MP、BI和ML方法，在定义集的SCI和MPI的基础上比较了拓扑意义。Coccinellinae、Epilachaninae 和 Scymninae（所有物种菌株）是单系的，而 Coccidulinae 和 Chilocorinae 的序列信息不足。Ortaliinae 和 Sticholotidinae，但尚未提交 ITS 序列。因此，对 ITS 区域结构折叠模式的分析提供了物种相关性和种内变异的进一步主要信息。Epilachaninae 和 Scymninae（所有物种菌株）是单系的，而 Coccidulinae 和 Chilocorinae 的序列信息不足。Ortaliinae 和 Sticholotidinae，但尚未提交 ITS 序列。因此，对 ITS 区域结构折叠模式的分析提供了物种相关性和种内变异的进一步主要信息。Epilachaninae 和 Scymninae（所有物种菌株）是单系的，而 Coccidulinae 和 Chilocorinae 的序列信息不足。Ortaliinae 和 Sticholotidinae，但尚未提交 ITS 序列。因此，对 ITS 区域结构折叠模式的分析提供了物种相关性和种内变异的进一步主要信息。