Chondroitin sulfate synthase-1 (ChSy-1) plays an important role in the synthesis and chain extension of chondroitin sulfate (CS). CS, a linear polysaccharide with high structural diversity, plays crucial roles in cell adhesion, morphogenesis and cell division in response to viral and bacterial infections. However, the role of ChSy-1 in the immune response remains unknown. In the present study, ChSy-1 from Pinctada fucata was identified. The deduced amino acid sequence of ChSy-1 contains a conserved domain, CHGN, which belongs to glycosyltransferase family A, and multiple sequence alignment revealed that CHGN shows evolutionary differences. Phylogenetic analysis indicated that ChSy-1 may be conserved evolutionarily. The nucleus grafting experiment showed that ChSy-1 possibly participates in wound repair and immune response to the graft. Lipopolysaccharide or polyinosinic:polycytidylic acid challenge and gene expression analysis especially suggested that ChSy-1 is involved in the immune response. The results of the present study indicate that ChSy-1 plays a role in the protection of the pearl oyster against infectious diseases.

中文翻译：

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硫酸软骨素合酶1基因在珍珠贝Pinctada fucata免疫反应中的作用

硫酸软骨素合酶-1 (ChSy-1) 在硫酸软骨素 (CS) 的合成和链延长中起重要作用。CS 是一种具有高度结构多样性的线性多糖，在响应病毒和细菌感染的细胞粘附、形态发生和细胞分裂中起着至关重要的作用。然而，ChSy-1 在免疫反应中的作用仍然未知。在本研究中，鉴定了来自 Pinctada fucata 的 ChSy-1。推导出的ChSy-1氨基酸序列包含一个保守结构域CHGN，属于糖基转移酶A家族，多序列比对显示CHGN存在进化差异。系统发育分析表明ChSy-1 在进化上可能是保守的。核移植实验表明ChSy-1可能参与创面修复和对移植物的免疫反应。脂多糖或聚肌苷酸：多胞苷酸攻击和基因表达分析尤其表明 ChSy-1 参与免疫反应。本研究的结果表明 ChSy-1 在保护珍珠贝免受传染病的侵害中发挥作用。