Background

Knowledge of local isolates and strains is a prerequisite for the development of either effective mass vaccination strategy or a suitable molecular marker-based diagnostic tool.

Purpose

The pathogenesis of Bovine tropical theileriosis (BTT), caused by Theileria annulata in susceptible ruminants, is known to vary depending upon the nature of isolate and strain involved. Therefore, RFLP and sequencing-based characterization of Indian isolates of T. annulata were attempted using TAMS gene.

Method

In the present study, TAMS 1 gene of T. annulata was amplified from 25 naturally infected calves from the BTT endemic semi-arid zone of Northern India. The amplified products were then digested with three restrictions enzymes viz., Taq I, Rsa I, and Alu I to find out the variations in pattern of restriction digests, so as to have an idea about the various isolates of T. annulata present in the studied area. Around 14 samples covering all the variants (from the PCR–RFLP patterns) were sequenced and submitted in NCBI (MH277607–MH277620).

Result

Coexistence of 4 variant genotypes was detected upon in-silico analysis of RFLP and sequence variations.

Conclusion

The nucleotide variations alongside the chromatogram analysis revealed point mutations leading to presence of noticeable genetic diversity among the isolates.

中文翻译：

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在半干旱印度新生儿犊牛中循环的多种环状泰勒虫基因型的共存。

背景

了解局部分离株和菌株是开发有效的大规模疫苗接种策略或合适的基于分子标记的诊断工具的先决条件。

目的

已知由易感反刍动物中的无环泰勒虫引起的牛热带泰勒虫病（BTT）的发病机理会根据所分离菌株和菌株的性质而有所不同。因此，RFLP和印度分离的基于测序的表征T.菇使用TAMS基因进行了尝试。

方法

在本研究中，TAMS 1基因T.菇是来自印度北部的BTT地方性半干旱地区25头自然感染的牛犊放大。然后，将扩增的产物用Taq I，Rsa I和Alu I这三种限制性酶消化，以找出限制性消化模式的变化，从而对存在于该环中的各种环孢菌有一个认识。研究区域。大约14个涵盖所有变异的样品（来自PCR-RFLP模式）已测序并提交NCBI（MH277607-MH277620）。

结果

在计算机上分析RFLP和序列变异后，检测到4个变异基因型的共存。

结论

色谱分析旁边的核苷酸变异揭示了点突变，导致分离株之间存在明显的遗传多样性。