Purpose

The aim of the present study was to detect and genotype T. gondii in free-range chickens destined to human consumption in Alagoas state, Brazil.

Methods

Two hundred blood samples were collected from free-range chickens and submitted to indirect immunofluorescence antibody test (IFAT). Brain tissue from 14 animals randomly selected were subjected to mouse bioassay. Positive samples in mouse bioassay were submitted to PCR and genotyped by PCR–RFLP.

Results

Out of two hundred blood samples from chickens, 72 (36%) samples were considered positive by IFAT. Two T. gondii strains were isolated, both being characterized as atypical and classified as #146 and a new genotype, named #279 in ToxoDB database.

Conclusions

Our results showed a sero-occurrence of T. gondii in free-range chickens intended for humans, and the genetic diversity of the parasite in Brazil, with a new genotype described.

中文翻译：

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在巴西供人类食用的散养鸡中出现非典型和新的弓形虫基因型。

目的

本研究的目的是检测巴西阿拉戈斯州供人类食用的散养鸡中的弓形虫并对其进行基因分型。

方法

从散养鸡身上采集了 200 份血样，并进行了间接免疫荧光抗体测试 (IFAT)。对随机选择的 14 只动物的脑组织进行小鼠生物测定。将小鼠生物测定中的阳性样品提交给 PCR 并通过 PCR-RFLP 进行基因分型。

结果

在来自鸡的 200 个血液样本中，72 个（36%）样本被 IFAT 认为是阳性的。分离出两种弓形虫菌株，均被定性为非典型并分类为 #146 和一种新的基因型，在 ToxoDB 数据库中命名为 #279。

结论

我们的研究结果表明，在供人类使用的散养鸡中，弓形虫的血清出现，以及巴西寄生虫的遗传多样性，并描述了一种新的基因型。