当前位置: X-MOL 学术Conserv. Genet. Resour. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Comparative analysis of microsatellite and SNP markers for parentage testing in the golden snub-nosed monkey ( Rhinopithecus roxellana )
Conservation Genetics Resources ( IF 1.1 ) Pub Date : 2020-04-03 , DOI: 10.1007/s12686-020-01147-7
Chen Ling , Wu Lixia , Hou Rong , Shen Fujun , Zhang Wenping , Tang Yao , Yuan Yaohua , Zhao Bo , Zhang Liang

Microsatellite markers are popular for assigning parentage, but single-nucleotide polymorphisms (SNPs) have only been applied in this area recently. To evaluate these two markers which have been previously studied in golden snub-nosed monkeys, we genotyped 12 individuals using 37 microsatellite loci and 37 SNP markers. The data showed that 32 of 37 microsatellite loci were polymorphic, and most microsatellite loci were high informative (mean PIC = 0.599). Meanwhile, 24 of 37 SNP markers were polymorphic and most were low informative (mean PIC = 0.244). For microsatellites, the combined exclusion probability with one-parent-unknown/known (CE-1P/CE-2P) nearly reached 1, while for the SNP markers, CE-2P only reached 0.9582. Under the condition of one parent known/unknown, the CE-2P and CE-1P could meet the international human parental standard (0.9973) by using five or nine microsatellite loci respectively. For SNP markers, we doubled the loci (n = 48) and simulated parentage testing, and the data showed that the CE-2P was 0.998 while the CE-1P was still low. This result indicated that the SNP loci which we used here had low polymorphism and that more loci need to be developed in the future. In addition, we corrected one case of failed identification by excluding siblings and reducing the range of candidate paternities.



中文翻译:

金丝鼻猴(Rhinopithecus roxellana)亲子鉴定中微卫星和SNP标记的比较分析

微卫星标记在分配亲缘关系方面很流行,但是单核苷酸多态性(SNP)只是最近才在这一领域应用。为了评估先前在金鼻鼻猴中研究的这两种标记,我们使用37个微卫星基因座和37个SNP标记对12个个体进行了基因分型。数据显示,在37个微卫星基因座中,有32个是多态性的,大多数微卫星基因座的信息量很高(平均PIC = 0.599)。同时,在37个SNP标记中,有24个是多态性的,大多数信息含量较低(平均PIC = 0.244)。对于微卫星,单亲未知/已知(CE-1P / CE-2P)的组合排除概率几乎达到1,而对于SNP标记,CE-2P仅达到0.9582。在一个已知/未知父母的条件下,CE-2P和CE-1P可以满足国际人类父母标准(0。9973)分别使用五个或九个微卫星基因座。对于SNP标记,我们将基因座(n = 48)加倍并进行了模拟亲子鉴定,数据显示CE-2P为0.998,而CE-1P仍然很低。该结果表明我们在此使用的SNP基因座具有低多态性,并且将来需要开发更多的基因座。此外,我们通过排除同级物并减小候选模式的范围来纠正了一种失败的识别情况。

更新日期:2020-04-03
down
wechat
bug