Numerous species from the classes Amphibia and Reptilia are facing extinction as a result of habitat loss and increasing anthropogenic impact on the environment; to be more specific, 32% amphibian and 20% reptilian species are either threatened or endangered. The aim of this review is to highlight the status of biobanking as one of the many tools within conservation of amphibians and reptilians. Moreover, we discuss opportunities and challenges as well as provide recommendations for future conservation and management strategies for saving vulnerable species from the two taxa. Previous studies have reported production of viable offspring from cryopreserved amphibian germplasm and in reptiles from cooled spermatozoa. However, further research is needed to improve the efficiency of cryopreserved germplasms for future use in biobanks. More advanced research directions include culture of fibroblast cell lines coupled with appropriate pluripotent stem cells and advanced reproductive technologies as this will represent one further unique and invaluable resource in species conservation and management.

两栖类和爬行类中的许多物种由于生境丧失和对环境的人为影响增加而面临灭绝；更具体地说，32％的两栖动物和20％的爬虫类动物受到威胁或濒危。这篇综述的目的是强调生物储备作为两栖动物和爬行动物保护中众多工具之一的地位。此外，我们讨论了机遇和挑战，并为未来的保护和管理策略提供了建议，以从这两个类别中拯救弱势物种。先前的研究报道了冷冻保存的两栖动物种质和冷却的精子在爬行动物中产生的后代。但是，需要进一步研究以提高冷冻保存的种质的效率，以备将来在生物库中使用。