We used double digest restriction site associated DNA sequencing (ddRAD) to develop new geographically informative nuclear SNP loci in Quercus robur and Quercus petraea. Genotypes derived from sequence data of 95 individuals covering the distribution range of the species were analysed to select geographically informative and polymorphic loci within Russia and Germany. We successfully screened a selected set of 119 loci on a MassARRAY® iPLEX™ platform on 190 individuals from 19 locations in Russia. The newly developed loci will be useful for genetic studies over the whole distribution range of both species.

中文翻译：

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在罗氏栎和栎属上开发新的SNP位点用于遗传研究，涵盖整个物种的分布范围

我们使用双酶切限制性酶切位点相关的DNA测序（ddRAD），以开发新的地理信息性核SNP位点，在栎和栎中。分析了来自覆盖该物种分布范围的95个个体的序列数据的基因型，以选择俄罗斯和德国的地理信息位点和多态位点。我们在MassARRAY®iPLEX™平台上成功筛选了来自俄罗斯19个地点的190个个体的119个基因座。新开发的基因座将对两个物种整个分布范围的遗传研究有用。