According to the most recent regulation, published in 2018, areas or states of Brazil where citrus canker is endemic are no longer obliged to eradicate citrus trees affected by the disease as in the past 60 years. Instead, growers have to adopt a set of control measures, such as copper sprays, windbreaks, and control of the citrus leaf miner to minimize the impact of the disease on fruit quality and yield. Another important change was that all fresh Fruit commercialized out of the state of origin and to other countries have to be sanitized against the canker-causing bacterium Xanthomonas citri subsp. citri ( X. citri ). Initially, sodium hypochlorite (NaOCl) was the only product allowed in Brazil by the referred legislation. Recently, this bactericide was prohibited to be used on fresh fruit shipped to the European Union and replaced by eugenol at 2%. Although effective, NaOCl may damage fruit skin, cause corrosion of packing house equipment and react with organic matter, which generates noxious by products. Here, we evaluated an alternative to NaOCl known as PosFruit. GC/MS and 1 H NMR chemical analyses showed that Posfruit contains both cinnamaldehyde isomers, with the trans being present in larger quantities. We showed that PosFruit was as effective as NaOCl to eliminate X. citri from citrus fruit artificially contaminated with the bacterium. In a pilot sanitization line, treatment with 2% PosFruit reduced the X. citri population on contaminated fruit by 4 log 10 CFU/mL. Furthermore, we detect neither the natural resistance of X. citri to PosFruit nor the persistence of the bacterium following progressive exposure to the product, which indicates that the product has multi-target action. PosFruit is a plant fortifier, residue-free, and efficient alternative to NaOCl for post-harvest decontamination of citrus fruit against X. citri .

中文翻译：

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一种基于肉桂醛的制剂作为次氯酸钠的替代品，用于柑橘类水果的采后净化

根据 2018 年发布的最新法规，巴西柑橘溃疡病流行的地区或州不再需要像过去 60 年那样根除受该病害影响的柑橘树。相反，种植者必须采取一套控制措施，例如喷铜、防风林和控制柑橘潜叶病，以尽量减少疾病对果实质量和产量的影响。另一个重要的变化是，所有在原产地和其他国家销售的新鲜水果都必须进行消毒，以防止引起溃疡病的细菌 Xanthomonas citri subsp。柑橘 (X. 柑橘)。最初，次氯酸钠 (NaOCl) 是巴西所提及立法允许的唯一产品。最近，这种杀菌剂被禁止在运往欧盟的新鲜水果上使用，并以 2% 的丁香酚代替。虽然有效，但 NaOCl 可能会损坏果皮，腐蚀包装厂设备并与有机物发生反应，产生有毒副产品。在这里，我们评估了一种名为 PosFruit 的 NaOCl 替代品。GC/MS 和 1 H NMR 化学分析表明 Posfruit 含有两种肉桂醛异构体，反式存在的量更大。我们证明 PosFruit 与 NaOCl 一样有效，可以从被细菌人工污染的柑橘类水果中清除柑橘类角霉。在中试消毒生产线中，使用 2% PosFruit 进行处理后，受污染水果上的柑橘溃疡病菌数量减少了 4 log 10 CFU/mL。此外，我们既没有检测到 X 的自然抗性。柑橘对 PosFruit 的影响，也没有在逐步接触产品后细菌的持续存在，这表明该产品具有多靶点作用。PosFruit 是一种植物强化剂，无残留，是 NaOCl 的有效替代品，用于柑橘类水果在收获后对柑橘叶斑病进行净化。