Temperature is a major environmental factor affecting the growth, development, and productivity of Sargassum fusiforme. We aimed to assess the metabolic processes and regulatory mechanisms in S. fusiforme during a 7-day high-temperature (27 °C and 32 °C) experiment. Changes in chlorophyll content and electrolyte leakage after high-temperature treatment were investigated. Metabolic changes in the leaves of S. fusiforme were analysed using gas chromatography–mass spectrometry. High temperatures suppressed chlorophyll content and increased electrolyte leakage. Further, a strong modulation of various metabolisms was observed: organic acids, amino acids, sugars or sugar alcohols, esters, and amines. These metabolisms were significantly enriched in ten pathways under the 27 °C treatment: aminoacyl-tRNA biosynthesis; glycine, serine, and threonine metabolism; alanine, aspartate, and glutamate metabolism; valine, leucine, and isoleucine biosynthesis; cyanoamino acid metabolism; cysteine and methionine metabolism; arginine and proline metabolism; tyrosine metabolism; citrate cycle (TCA cycle); and glucosinolate biosynthesis. The various metabolisms significantly enriched seven pathways under the 32 °C treatment, namely, alanine, aspartate, and glutamate metabolism; aminoacyl-tRNA biosynthesis; phenylalanine metabolism; tyrosine metabolism; arginine and proline metabolism; nitrogen metabolism; and isoquinoline alkaloid biosynthesis. These changes in metabolic pathways may contribute to the tolerance and adaptability of S. fusiforme to high-temperature stress.

中文翻译：

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热胁迫对羊栖菜叶代谢产物的影响

温度是影响羊栖菜（Sargassum fusiforme）生长，发育和生产力的主要环境因素。我们的目的是在7天的高温（27°C和32°C）实验中评估镰形葡萄球菌的代谢过程和调控机制。研究了高温处理后叶绿素含量和电解质泄漏的变化。梭状芽孢杆菌叶片的代谢变化使用气相色谱-质谱法分析e。高温抑制叶绿素含量并增加电解质泄漏。此外，观察到各种新陈代谢的强烈调节：有机酸，氨基酸，糖或糖醇，酯和胺。在27°C处理下，这些代谢显着丰富于十个途径：氨酰基-tRNA生物合成；甘氨酸，丝氨酸和苏氨酸代谢；丙氨酸，天冬氨酸和谷氨酸代谢；缬氨酸，亮氨酸和异亮氨酸的生物合成；氰基氨基酸代谢；半胱氨酸和蛋氨酸的代谢；精氨酸和脯氨酸代谢；酪氨酸代谢 柠檬酸盐循环（TCA循环）；和芥子油苷的生物合成。在32°C处理下，各种新陈代谢显着丰富了七个途径，即丙氨酸，天冬氨酸和谷氨酸代谢。氨酰基-tRNA的生物合成；苯丙氨酸代谢 酪氨酸代谢 精氨酸和脯氨酸代谢；氮代谢 和异喹啉生物碱的合成。这些代谢途径的改变可能有助于梭状芽孢杆菌能承受高温胁迫。