Recent extensive studies of long non-coding RNAs (lncRNAs) have shown that lncRNAs play an important role in plant development and growth. Populus euphratica Oliv. is the only natural tree species in the Taklimakan desert with leaves that change shapes at different growth stages. LncRNAs can affect biological functions in P. euphratica heterophyllous morphogenesis, but there are few reports on this subject in the literature. In this study, chain-specific transcriptome sequencing and small RNA sequencing were performed using linear, lanceolate, broad-ovate and ovoid leaves of P. euphratica. A total of 442 novel P. euphratica lncRNAs were predicted and the expression profiles of 4460 lncRNAs were analyzed. 371 lncRNAs were significantly different among the varied leaf shapes studied. Using co-expression analysis and according to the principle of competing endogenous RNA (ceRNA), we found that 28 lncRNAs could regulate the expression of 133 mRNAs by antagonizing 17 miRNAs. These mRNAs are involved in 23 biological functions, such as cell growth, developmental processes, metabolic processes and biological regulation, thereby affecting the formation of P. euphratica heteromorphic leaves. These results provide the foundation to study the formation of P. euphratica heteromorphic leaves, revealing the function of lncRNAs in the development of leaves in plants.

中文翻译：

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胡杨的起源中长非编码RNA的全基因组鉴定和功能预测。异形叶

对长非编码RNA（lncRNA）的近期广泛研究表明，lncRNA在植物发育和生长中起重要作用。胡杨。是塔克拉玛干沙漠中唯一的自然树种，其叶子在不同的生长阶段会发生变化。LncRNA可以影响胡杨假单胞菌形态发生中的生物学功能，但是在文献中对此主题的报道很少。在这项研究中，使用胡杨假单胞菌的线性，披针形，宽卵形和卵形叶片进行链特异性转录组测序和小RNA测序。共有442篇胡杨假单胞菌预测了lncRNA，并分析了4460个lncRNA的表达谱。在研究的各种叶片形状中，有371个lncRNA明显不同。使用共表达分析，并根据竞争内源性RNA（ceRNA）的原理，我们发现28个lncRNAs可以通过拮抗17个miRNA来调节133个mRNA的表达。这些mRNA参与23种生物学功能，例如细胞生长，发育过程，代谢过程和生物学调节，从而影响胡杨假单胞菌异形叶的形成。这些结果为研究胡杨假单胞菌异形叶的形成提供了基础，揭示了lncRNA在植物叶片发育中的功能。