As an important legume plant, Medicago truncatula is a preeminent model for the study of the processes of nitrogen fixation, symbiosis, and legume genomics. The regulatory mechanism of the cytokinin response has been studied in many plants, such as rice, Arabidopsis, tomato, and barley, but information about regulatory pathways and genes involved in the cytokinin response in Medicago truncatula is notably limited. In this study, to better understand the cytokinin response in Medicago truncatula, transcriptome analysis of seedlings grown with 6-benzylaminopurine or lovastatin was performed using RNA-Seq. In this study, 3627 and 3093 transcripts were differentially expressed in cytokinin-induced/control (Cyto/Ctrl) and cytokinin-inhibited/control (Inh/Ctrl) groups, respectively, and differentially expressed genes were tested by quantitative real-time PCR (qRT-PCR). Analysis of the cytokinin response in Medicago truncatula revealed a large number of transcripts involved in signal transduction, metabolic process, secondary metabolite biosynthesis, transport and catabolism, growth and development, defense mechanisms, and transcription. There were 43 transcription factor families, including 1845 transcription factor (TF) genes with 2147 TF transcripts, as detected by RNA-Seq. Additionally, 216 TF genes with 220 transcripts were differentially expressed in Cyto/Ctrl, and 185 TF genes with 189 transcripts were in Inh/Ctrl. A total of 289 and 260 DETs involved in biosynthesis, metabolism, and transduction of plant hormones were identified in the Cyto/Ctrl and Inh/Ctrl groups, respectively. Furthermore, 15 transcripts, including A-ARR, IPT, and CKX, were demonstrated to play roles in cytokinin regulation or signal transduction. These findings were associated with the cytokinin response in other plants. The resulting data provide the first cytokinin transcriptome analysis in Medicago truncatula. Further analysis and identification of cytokinin-regulated transcripts or signal transduction transcripts may help to elucidate the regulatory mechanisms governing the cytokinin response in Medicago truncatula.

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Medi藜苜蓿细胞分裂素应答的转录组分析

作为重要的豆科植物，run藜苜蓿是研究固氮，共生和豆科植物基因组学过程的杰出模型。已经在许多植物中研究了细胞分裂素应答的调节机制，例如水稻，拟南芥，番茄和大麦，但是关于t藜苜蓿中涉及细胞分裂素应答的调节途径和基因的信息明显有限。在这项研究中，为了更好地了解截叶苜蓿中的细胞分裂素反应用RNA-Seq对6-苄基氨基嘌呤或洛伐他汀生长的幼苗进行转录组分析。在这项研究中，3627和3093转录本分别在细胞分裂素诱导/对照（Cyto / Ctrl）和细胞分裂素抑制/对照（Inh / Ctrl）组中差异表达，并通过定量实时PCR检测差异表达的基因（ qRT-PCR）。苜蓿中细胞分裂素反应的分析揭示了大量与信号转导，代谢过程，次生代谢产物生物合成，转运和分解代谢，生长与发育，防御机制和转录有关的转录本。RNA-Seq检测到，共有43个转录因子家族，包括1845个转录因子（TF）基因和2147个TF转录本。此外，在Cyto / Ctrl中差异表达了216个TF基因和220个转录物，在Inh / Ctrl中差异化了185个TF基因和189个转录物。在Cyto / Ctrl和Inh / Ctrl组中分别鉴定出涉及植物激素的生物合成，代谢和转导的总共289和260个DET。此外，包括A-ARR，IPT和CKX在内的15种转录物被证明在细胞分裂素调节或信号转导中起作用。这些发现与其他植物中的细胞分裂素反应有关。所得数据提供了第一个细胞分裂素转录组分析紫花苜蓿。进一步分析和鉴定细胞分裂素调控的转录本或信号转导转录本可能有助于阐明截短苜蓿中细胞分裂素应答的调控机制。