Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici (Pst), is considered as a major threat to the wheat production worldwide, including Pakistan. In the current study, 80 wheat landraces collected from different agroecological zones of Pakistan were screened for their stripe rust resistance. Molecular markers were used to identify/postulate the stripe rust genes in these landraces. The results indicated that 46 (57.5%) landraces had all-stage resistance, and only 4 (5%) landraces showed adult-plant resistance. The possible stripe rust resistance genes in these entries were postulated by the using already reported linked molecular markers of all-stage resistance and adult plant stage resistance genes (Yr4 Yr5, Yr10, Yr15 and Yr24/26 and adult-plant resistance genes Yr18, Yr29 and Yr32). Molecular analysis indicated that resistant genes Yr4, Yr5, Yr10, Yr15, Yr17, Yr18, Yr26/24, Yr29 and Yr32 were found in 16(20%), 12(15%), 4(5%), 15(18.75%), 16(20%), 13(16.25%), 39(48.75%), 20(25%) and 7(7.5%) of landraces, respectively. Majority of the landraces showed the presence of more than one gene. These lines could be used effectively in the wheat breeding programs.

条锈病（黄色锈病），由条锈菌F。sp。Tritici（Pst）被认为是对包括巴基斯坦在内的全球小麦生产的主要威胁。在当前的研究中，对从巴基斯坦不同农业生态区收集的80个小麦地方品种进行了筛选，以了解它们的抗条锈性。分子标记用于鉴定/推测这些地方种中的条锈基因。结果表明，有46种（57.5％）的地方品种具有全阶段抗性，只有4种（5％）的地方品种具有成年植物抗性。这些条目中可能的条纹锈病抗性基因是通过使用已经报道的所有阶段抗性和成年植物阶段抗性基因的连锁分子标记（Yr 4Yr5，Yr10，Yr15和Yr24 / 26以及成年植物抗性基因Yr18，Yr29和Yr32）。分子分析表明，抗性基因YR 4，YR5，Yr10，Yr15，Yr17，Yr18，Yr26 / 24，YR 29和YR 32 16（20％）被发现，12（15％），4（5％），15 （18.75％），16（20％），13（16.25％），39（48.75％），20（25％）和7（7.5％）的地方品种。多数地方品种显示存在多个基因。这些品系可以有效地用于小麦育种计划。