Porcine epidemic diarrhea (PED) is a devastating enteric disease causing economic losses in many countries including the Philippines. To control PED, apart from oral administration of minced intestinal materials, there are still no effective control methods. The ability of porcine epidemic diarrhea vaccine RNA particle platform (PED-RP) to induce antibody in colostrum and milk samples was investigated in two pig herds with a differing PED status in the Philippines. Herd-A was naïve but herd-B was endemically infected with PED. Sera, colostrum, and milk samples were collected prior to and following vaccination, and assayed for the presence of antibody by viral neutralization (VN) and IgG and IgA levels by ELISA spike protein. The results from both herds, compared to the non-vaccinated control group, demonstrated significantly increased VN titers and IgG and IgA levels in colostrum and milk samples of sows at 0, 7, 14, and 21 days post parturition. Additionally, piglets from vaccinated sows had VN titers, and IgG and IgA levels are significantly higher than those from non-vaccinated sows. In conclusion, the results of the study demonstrate that PED-RP can be used to induce a satisfactory antibody response in colostrum and milk, as measured by VN titers and IgG and IgA levels.

猪流行性腹泻（PED）是一种破坏性肠病，在包括菲律宾在内的许多国家中造成了经济损失。为了控制PED，除了口服切碎的肠材料外，尚无有效的控制方法。在菲律宾的两个猪群中，PED状况不同，研究了猪流行性腹泻疫苗RNA颗粒平台（PED-RP）在初乳和牛奶样品中诱导抗体的能力。牛群A幼稚，但牛群B普遍感染PED。在疫苗接种之前和之后，收集血清，初乳和牛奶样品，并通过病毒中和（VN）和ELISA穗状蛋白测定IgG和IgA水平来检测抗体的存在。与未接种疫苗的对照组相比，两种牛群的结果，在分娩后0、7、14和21天，母猪初乳和牛奶样品中的VN滴度，IgG和IgA水平显着提高。此外，接种疫苗的母猪的仔猪具有VN滴度，并且IgG和IgA水平明显高于未接种疫苗的母猪。总之，该研究结果表明，如VN滴度，IgG和IgA水平所测，PED-RP可用于在初乳和牛奶中诱导令人满意的抗体反应。