The effect of hemoglobin polymorphism on performance traits in Nigerian indigenous chicken types was investigated in this research. The chickens were obtained through the pure mating of the sire and dam of each population of frizzle feathered, normal feathered, and naked neck chickens to produce F₁ offsprings. One hundred fifty-five chicks (37 frizzle, 79 normal, and 39 naked neck) were measured for body weight (g), breast girth (cm), and tibia length (cm). At 20 weeks, 5 mL of blood was collected from the wing vein of each chicken into heparinized tubes and labeled according to its tag number for electrophoresis. The electrophoresis procedures outlined by RIKEN BRC (2006) was used. 0.6 μl of undiluted blood constituent was taken. The prepared buffer (Tris 10.91 g, EDTA 0.60 g, boric acid 3.10 g) at pH 8.5 was used for the gel preparation. The cellulose acetate membrane was used as a supporting medium. The electrophoresis was carried out at a voltage of 150 V for about 50 min at a temperature of 4 °C. The migration of the genotype was from cathode (−) to anode (+). Each bird was scored as either fast (AA), midway (AB), or slow (BB) according to the mobility on the cellulose acetate paper for hemoglobin. Data were analyzed using statistical analysis system (SAS 2002), and significant means were separated using Tukey’s Honestly Significant Difference. Hardy Weinberg’s equation was used to calculate genotypic and allelic frequencies and tested using chi-square (χ²). Hemoglobin had three polymorphic forms viz AA, AB, and BB. The genotypic frequencies of hemoglobin (Hb^AA, Hb^AB, and Hb^BB) were 49, 56, and 50, respectively, while the allelic frequencies were 0.50 for both Hb^A and Hb^B. The effect of the polymorphic forms on body weight (g), breast girth (cm), and tibia length (cm) showed that the AA had significantly higher (P < 0.05) body weight (g) than AB and BB (1296.43 g, 1029.59 g, and 884.46 g, respectively). The AA was also higher (P < 0.05) than the AB and BB for breast girth (cm) and tibia length (cm). Heterozygotes adapted and survived better than the homozygotes. The effect of the polymorphic forms of hemoglobin on body weight (g), breast girth (cm), and tibia length (cm) showed that it could be used for body weight selection.

在这项研究中，研究了血红蛋白多态性对尼日利亚本土鸡类型的性能特征的影响。这些鸡是通过每只毛茸茸，普通羽毛和裸颈鸡的种群的父系和母系的纯交配获得F _1的后代。测量了一百五十五只小鸡（37毛躁，79正常和39裸露的脖子）的体重（g），胸围（cm）和胫骨长度（cm）。在20周时，从每只鸡的翼静脉收集5 mL血液到肝素化管中，并根据其标记号进行电泳标记。使用了RIKEN BRC（2006）概述的电泳程序。取0.6μl未稀释的血液成分。将pH为8.5的制备的缓冲液（Tris 10.91g，EDTA 0.60g，硼酸3.10g）用于凝胶制备。乙酸纤维素膜用作支撑介质。在150℃的电压下在4℃的温度下进行电泳约50分钟。基因型的迁移是从阴极（-）到阳极（+）。每只鸟均被评为快（AA），中途（AB），根据醋酸纤维素纸上血红蛋白的迁移率，选择慢速（BB）。使用统计分析系统（SAS 2002）对数据进行分析，并使用Tukey的“诚实显着性差异”将重要的方法分开。Hardy Weinberg方程用于计算基因型和等位基因频率，并使用卡方（χ ²）。血红蛋白具有三种多态形式，即AA，AB和BB。血红蛋白（Hb ^AA，Hb ^AB和Hb ^BB）的基因型频率分别为49、56和50，而Hb ^A和Hb ^B的等位基因频率均为0.50 。多态形式对体重（g），胸围（cm）和胫骨长度（cm）的影响表明，AA的 体重（g）显着高于AB和BB（1296.43 g，P <0.05），分别为1029.59克和884.46克）。AA也更高（P <0.05）的胸围（cm）和胫骨长度（cm）大于AB和BB。杂合子比纯合子适应和生存更好。血红蛋白的多态形式对体重（g），胸围（cm）和胫骨长度（cm）的影响表明，它可用于体重选择。