Purpose

The present study focused on synthesis and characterization of polymeric nanocarrier for the hydrophobic drug, naringenin (Nar), using chitosan (CS) and dextran sulfate (DS).

Method

CSDS-Nar and blank CSDS nanoparticles were prepared by complex coacervation technique. The nanoparticles were characterized by scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FTIR), X-ray diffraction (XRD), and dynamic light scattering (DLS). Cytotoxicity evaluation of blank CSDS and CSDS-Nar was performed by MTT assay after 24-h incubation.

Result

The nanoparticles were observed to have spherical morphology. The size and zeta potential of the CSDS-Nar were ~ 337.2 ± 48.27 nm and − 34.4 ± 7.45 mV, respectively. The interactions between polymer and drug were confirmed by FTIR studies. The in vitro drug release studies showed that 80% of free naringenin was released rapidly at 36 h. On the other hand, 51% naringenin was released from CSDS-Nar at 36 h. MTT assay demonstrated that at higher dose, the cell viability of MCF-7 cells was 45% and 8% after CSDS and CSDS-Nar treatment, respectively.

Conclusion

Hence, the empirical findings of the study suggest that CSDS nanocarrier could be utilized as a promising and ideal carrier for delivery of naringenin and similar hydrophobic drugs to cancer cells.

中文翻译：

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柚皮素-壳聚糖-葡聚糖硫酸盐纳米载体的合成与表征

目的

本研究致力于使用壳聚糖（CS）和硫酸葡聚糖（DS）合成和表征疏水性药物柚皮苷（Nar）的聚合物纳米载体。

方法

通过复合凝聚技术制备了CSDS-Nar和空白CSDS纳米粒子。通过扫描电子显微镜（SEM），傅立叶变换红外光谱（FTIR），X射线衍射（XRD）和动态光散射（DLS）对纳米颗粒进行表征。孵育24小时后，通过MTT分析对空白CSDS和CSDS-Nar进行细胞毒性评估。

结果

观察到纳米颗粒具有球形形态。CSDS-Nar的大小和Zeta电位分别约为337.2±48.27 nm和− 34.4±7.45 mV。FTIR研究证实了聚合物与药物之间的相互作用。体外药物释放研究表明，80％的游离柚皮素在36 h迅速释放。另一方面，CSDS-Nar在36 h释放了51％的柚皮素。MTT分析表明，在CSDS和CSDS-Nar处理后，在较高剂量下，MCF-7细胞的细胞活力分别为45％和8％。

结论

因此，该研究的经验发现表明CSDS纳米载体可以用作将柚皮苷和类似疏水性药物递送至癌细胞的有希望的理想载体。