An efficient in vitro propagation and synthetic seed production protocol was established for the conservation of Decalepis salicifolia (Bedd. ex Hook.f.) Venter, an endemic and critically endangered ethnomedicinal species. Murashige and Skoog (MS) media supplemented with 2.2 μM BAP and 5.7 μM IAA produced shoot tip proliferation with an average shoot length of 5.81 ± 0.03 cm in 3 wk. Axillary bud proliferation was found to be best in 11.1 μM BAP with an average of 3.5 ± 0.06 shoots per explant. Shoot tip and nodal explants were encapsulated in sodium alginate and their regeneration was achieved following storage at 4°C up to 12 wk. Successful rooting was obtained in modified MS medium with low nitrate and high sucrose concentration. Quarter strength MS media containing 2.5 mM each of NH₄NO₃ and KNO₃ along with 8% sucrose produced an average number of 12.4 ± 1.18 roots with an average length of 4.3 ± 0.08 cm. The in vitro derived rooted plants were successfully hardened (92.8%) and established in field with 100.0% survival rate. Inter-simple sequence repeat (ISSR) markers proved the genetic fidelity among the in vitro grown plant showing 99.5% similarity with the mother plant. Micropropagation derived acclimatized plants produced 2-hydroxy-4-methoxybenzaldehyde in amounts similar to seed-derived field-grown plants of the same age. The in vitro propagation protocol developed for D. salicifolia can be utilized to reduce exploitation pressure from their natural habitats and augment ecorestoration, conservation, and cultivation of this critically endangered and industrially valuable plant. The synthetic seeds technique will serve as propagules in ex situ gene banks, as well as supports cost-effective germplasm conservation.

建立了一种有效的体外繁殖和合成种子生产方案，以保护十足小Dec（Bedd。ex Hook.f.）Venter，一种地方性的和极度濒临灭绝的人种医学物种。补充有2.2μMBAP和5.7μMIAA的Murashige和Skoog（MS）培养基在3周内产生枝条尖端增殖，平均枝条长度为5.81±0.03 cm。发现腋芽的增生最好在11.1μMBAP中，每个外植体平均有3.5±0.06芽。将芽尖和节外植体封装在藻酸钠中，并在4°C储存至12 wk后实现再生。在硝酸盐含量低且蔗糖浓度高的改良MS培养基中成功生根。四分之一强度MS介质，每个介质均包含2.5 mM NH ₄ NO ₃和KNO ₃连同8％的蔗糖产生的平均根数为12.4±1.18，平均长度为4.3±0.08 cm。在体外衍生生根的植物被成功硬化（92.8％）和100.0％的存活率成立于现场。简单序列间重复（ISSR）标记证明了体外生长植物的遗传保真度，与母体植物有99.5％的相似性。微繁殖衍生的驯化植物产生的2-羟基-4-甲氧基苯甲醛的量与相同年龄的种子衍生的田间种植植物相似。在体外用于开发传播协议D.柳叶可用于减轻其自然栖息地的开发压力，并增强这种极度濒危和工业上有价值的植物的生态修复，保护和种植。合成种子技术将在异地基因库中用作繁殖体，并支持经济高效的种质保护。