Randia echinocarpa, an endemic plant to Northwest Mexico, is used as food and in traditional medicine, and several of its biological activities have been demonstrated (antioxidant, antimutagenic, antidiabetic, and immunomodulatory). Plant tissue culture is a safe and scalable system for plant propagation and production of bioactive compounds. Therefore, this study aims to establish protocols for seed germination and callus culture of R. echinocarpa and to evaluate the antioxidant activity of methanol extracts (ME) of plantlets and calli via the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) methods. Seeds were cultured in media with different concentrations of Murashige and Skoog (MS) salts and sucrose, and a higher germination rate and plantlet growth was observed in half-strength MS medium with 15 g L⁻¹ of sucrose. Calli were obtained from cotyledon and hypocotyl explants cultured in MS media with different concentrations of benzyl aminopurine (BAP) and indole-3-acetic acid (IAA). All treatments induced callus formation in 100% of explants; however, the medium containing 1 mg L⁻¹ BAP + 1 mg L⁻¹ IAA was selected because it produced calli with higher biomass and friable texture. The ME of cotyledons showed the highest antioxidant activity values (μmol Trolox per 100 g dry weight) in DPPH (345.5) and ABTS (1166.4) assays, whereas the ME of calli from hypocotyls showed a higher antioxidant activity than the ME of calli from cotyledons in both antioxidant assays. The tissue culture protocols established here will be useful for R. echinocarpa germplasm conservation and propagation, as well as for the production of bioactive compounds.

Randia echinocarpa是墨西哥西北部的特有植物，被用作食品和传统医学，并且已经证明了其一些生物学活性（抗氧化，抗诱变，抗糖尿病和免疫调节）。植物组织培养是用于植物繁殖和生产生物活性化合物的安全且可扩展的系统。因此，本研究的目的是建立用于种子萌发和愈伤组织培养的协议R. echinocarpa并评价植株和愈伤组织的甲醇提取物（ME）的抗氧化活性通过2,2-二苯基-1-吡啶并肼（DPPH）和2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）方法。在具有不同浓度的Murashige和Skoog（MS）盐和蔗糖的培养基中培养种子，在含15 g L ^-1蔗糖的半强度MS培养基中观察到更高的发芽率和幼苗生长。从在具有不同浓度的苄基氨基嘌呤（BAP）和吲哚-3-乙酸（IAA）的MS培养基中培养的子叶和下胚轴外植体中获得愈伤组织。所有处理均会在100％的外植体中诱导愈伤组织形成；但是，含有1 mg L ^-1 BAP + 1 mg L ^-1的培养基选择IAA是因为它能产生具有更高生物量和易碎质地的愈伤组织。在DPPH（345.5）和ABTS（1166.4）分析中，子叶的ME显示出最高的抗氧化活性值（每100克干重μmolTrolox），而下胚轴的愈伤组织的ME则比子叶的愈伤组织的ME具有更高的抗氧化活性。在两种抗氧化剂测定中。此处建立的组织培养规程将对棘皮癣菌种质的保存和繁殖以及生物活性化合物的生产有用。