We examined the effects of culture conditions, namely, illumination, temperature and the addition of 6-benzylaminopurine (BAP) to the germination medium, on the asymbiotic germination of mature seeds of Gastrodia pubilabiata Sawa, an achlorophyllous orchid. Mature seeds were harvested from plants at two sites, one in Toyama Prefecture and one in Chiba Prefecture, Japan. We used gellan gum-solidified New Dogashima medium as the basal medium. The maximum frequency of germination of the seeds in darkness was approximately 65%. BAP had no stimulatory effect on seed germination, but accelerated protocorm development. Necessary conditions for successful germination of seeds and subsequent development of seedlings from mature seeds of Gastrodia pubilabiata clarified in this study are prerequisites not only for conservation of biodiversity but also for the elucidation of the life cycle of this achlorophyllous orchid.

我们检查了培养条件，即光照，温度以及向发芽培养基中添加6-苄基氨基嘌呤（BAP）对无叶兰花天麻成熟种子非共生萌发的影响。从两个地点的植物中收获成熟种子，一个地点在日本富山县，另一个地点在日本千叶县。我们使用结冷胶固化的新多谷岛培养基作为基础培养基。黑暗中种子发芽的最大频率约为65％。BAP对种子发芽没有刺激作用，但能促进原球茎发育。天麻成熟种子成功发芽和幼苗发育的必要条件 在这项研究中阐明，不仅是保护生物多样性的前提，而且是阐明这种无叶兰花的生命周期的前提。