Propagation of gametophytes and sporophytes using mechanical fragmentation has been considered a suitable method for mass production of ferns. This study aimed to develop a practical propagation method for Lemmaphyllum microphyllum C. Presl, which is a fern of significant ornamental and medicinal value. Gametophytes were obtained through in vitro spore germination and used for propagation experiments. The gametophyte was mechanically fragmented using a scalpel into small fragments, which were then used to investigate gametophyte proliferation. In addition, the gametophyte was fragmented using a blender and then used to study sporophyte formation. Optimal proliferation conditions of the gametophyte were determined using Murashige and Skoog (MS) basal medium (double-, full-, half-, quarter-strength), Knop medium, and medium components (sucrose, nitrogen sources, activated charcoal), at various concentrations. The fresh weight of the gametophyte was 14-fold higher than that of gametophytes (300 mg) used as culture material, when cultured on double-strength MS. Moreover, 1 g of the gametophyte fragmented in 25 mL of distilled water formed more than 430 sporophytes in a soil mixture in an area of 7.5 cm². The sporophytes were successfully cultivated in the greenhouse after acclimation. A large-scale production method for L. microphyllum that can be easily implemented in a fern production farm is outlined.

使用机械裂解繁殖配子体和孢子体被认为是大量生产蕨类植物的合适方法。这项研究的目的是开发一种实用的繁殖方法，用于小叶小球藻（P. Lemmaphyllum C. Presl），这是一种具有重要观赏和药用价值的蕨类植物。配子体通过体外获得孢子萌发并用于繁殖实验。用解剖刀将配子体机械破碎成小碎片，然后将其用于研究配子体增殖。另外，使用搅拌器将配子体破碎，然后用于研究孢子体的形成。使用Murashige和Skoog（MS）基础培养基（双倍，全倍，半倍，四分之一强度），Knop培养基和中等成分（蔗糖，氮源，活性炭）确定配子体的最佳增殖条件。浓度。当在双强度MS上培养时，配子体的新鲜重量比用作培养材料的配子体（300 mg）高14倍。此外，²。适应后，将孢子体成功地在温室中栽培。概述了可以在蕨类植物生产场中轻松实施的小叶李斯特菌的大规模生产方法。