To produce malic acid from non-oxidative pathway route in Escherichia coli using two key enzymes and synthetic scaffold complex. E. coli was engineered to produce malic acid from glucose by co-localization of two key enzymes phosphoenolpyruvate carboxylase (Ppc) and malate dehydrogenase (MdhA) with synthetic scaffold complex. Scaffold plasmid has produced the maximum concentration of 3.51 g/L malic acid from 10 g/L glucose in 48 h of culture. pH 5.5 and temperature 30°C were optimum for malic acid production without any engineering of competing metabolic pathways. E. coli mutant strains and different concentrations of glucose also tested. When 50 g/L glucose was used as substrate, 20.4 g/L of malic acid was produced.

使用两种关键酶和合成支架复合物从大肠杆菌的非氧化途径途径生产苹果酸。通过将两种关键酶磷酸烯醇丙酮酸羧化酶（Ppc）和苹果酸脱氢酶（MdhA）与合成支架复合物共定位，对大肠杆菌进行了工程改造，以从葡萄糖生产苹果酸。在48小时的培养中，支架质粒从10 g / L葡萄糖中产生的最大浓度为3.51 g / L苹果酸。pH 5.5和温度30°C最适合生产苹果酸，而无需任何竞争性代谢途径的工程设计。还测试了大肠杆菌突变菌株和不同浓度的葡萄糖。当使用50g / L葡萄糖作为底物时，产生了20.4g / L苹果酸。