2,3-Butanediol (2,3-BDO) is a promising bio-based chemical for its wide range of applications in industrial areas as synthetic rubber precursor, food additives, and cosmetics. In this study, Escherichia coli DH5α was metabolically engineered for enhanced production of 2,3-BDO by expressing Bacillus subtilis alsS, alsD, and ydjL genes encoding α-acetolactate synthase, α-acetolactate decarboxylase, and acetoin reductase/2,3 -butanediol dehydro¬genase, respectively, along with Deinococcus radiodurans dr1558 gene encoding a response regulator. When recombinant E. coli DH5α strain expressing only B. subtilis alsS, alsD, ydjL genes was cultured in LB medium containing 20 g/L glucose, 3.14 g/L of 2,3-BDO was produced. Additional expression of D. radiodurans dr1558 gene in E. coli DH5α expressing alsS, alsD, and ydjL genes resulted in the production of 7.81 g/L of 2,3-BDO under the same culture conditions, which is 2.5 fold higher than that produced by the strain without DR1558. Transcriptional analysis of E. coli DH5α expressing DR1558 suggested that the expression levels of the genes related to 2,3-BDO pathways were enhanced, while those of genes related to by-product pathways were suppressed, compared with control strain expressing only 2,3-BDO synthesis genes. These results strongly suggest that introduction of the stress tolerant response regulator DR1558 can modulate metabolic pathways to favor production of the target product.

2,3-丁二醇（2,3-BDO）是一种有前途的生物基化学品，由于其在工业领域的广泛应用，包括合成橡胶前体，食品添加剂和化妆品。在这项研究中，大肠杆菌DH5α经过代谢工程改造，通过表达枯草芽孢杆菌alsS，alsD和ydjL基因编码α-乙酰乳酸合酶，α-乙酰乳酸脱羧酶和乙酰还原酶/ 2,3-丁二醇来增强2,3-BDO的产生。脱氢酶以及放射性杜鹃球菌dr1558基因编码反应调节剂。当重组大肠杆菌DH5α菌株仅表达枯草芽孢杆菌alsS，alsD，在含有20 g / L葡萄糖的LB培养基中培养ydjL基因，产生3.14 g / L的2,3-BDO。的附加表达耐辐射球菌dr1558在基因大肠杆菌表达DH5α的alsS，alsD和ydjL基因导致在相同的培养条件下生产7.81克/升2,3-BDO的，这是2.5比产生倍高通过没有DR1558的菌株。大肠杆菌的转录分析与仅表达2,3-BDO合成基因的对照菌株相比，表达DR1558的DH5α表明与2,3-BDO途径相关的基因的表达水平提高，而与副产物途径相关的基因的表达水平被抑制。这些结果强烈表明，引入胁迫耐受反应调节剂DR1558可以调节代谢途径，从而有利于目标产品的生产。