Microbial cellulases have become the mainstream biocatalysts due to their complex nature and widespread industrial applications. Here, homogeneous endoglucanase GluCB31 from Bacillus subtilis subsp. inaquosorum CBS31 was studied. GluCB31 was purified to 17.68-fold with an 8.33% yield and a specific activity of 1066.37 U/mg. Biochemical properties of GluCB31 were performed and the results are as follows; molecular mass of 35 kDa with an optimum pH at 7.5 and temperature at 50°C. GluCB31 was immobilized in calcium alginate gel and it exhibited the highest activity at 10°C higher temperature than soluble enzyme, as the entrapment in alginate gel made GluCB31 more stable. Kinetic studies showed the V_max of 1293.33 ± 2.51 U/mg and K_m of 0.0183 mg/mL. Enzymatic activity was activated by Tween-20 (106.7%), Tween-80 (111.6%), Triton X-100 (142.3%), SDS (135.5%), Mg⁺⁺ (185.7%), Cu⁺⁺ (167.6%), Zn⁺⁺ (153.7%), Mn⁺⁺ (106.3%), Ba⁺⁺ (181.9%), Ni⁺⁺ (107.2%) while inhibited by Fe⁺⁺ (15.8%), β-mercaptoethanol (46.8%), EDTA (54.5%). Enthalpy, free energy, and entropy of activation were calculated to be 38.526 kJmol^-1, 44.187 kJmol^-1, and -17.518 Jmol^-1K^-1 respectively. Also, ΔG_E-S and ΔG_E-T were found to be -10.75 kJmol^-1 and -45.92 kJmol^-1 respectively. A low ΔS, ΔG_E-S, and ΔG_E-T values were signified enzyme-catalyzed reaction occurs at a fast rate and the existence of the enzyme in its stable state. Cellobiose was the major end product of hydrolysis. These attributes of GluCB31 demonstrated the diversity of catalytic activities and serve in various biotechnological processes, thus deserve to be developed as a bio-industrial agent.

微生物纤维素酶由于其复杂的性质和广泛的工业应用已成为主流的生物催化剂。在这里，来自枯草芽孢杆菌亚种的均质内切葡聚糖酶GluCB31 。研究了inaquosorum CBS31。GluCB31纯化至17.68倍，收率8.33％，比活性为1066.37 U / mg。进行了GluCB31的生化特性分析，结果如下。分子量为35 kDa，最适pH为7.5，温度为50°C。GluCB31被固定在海藻酸钙凝胶中，并且在10°C的温度下比可溶性酶具有最高的活性，这是因为海藻酸盐凝胶中的截留使GluCB31更稳定。动力学研究表明V _max为1293.33±2.51 U / mg和K _m0.0183 mg / mL。Tween-20（106.7％），Tween-80（111.6％），Triton X-100（142.3％），SDS（135.5％），Mg ⁺⁺（185.7％），Cu ⁺⁺（167.6％）激活了酶活性。），Zn ⁺⁺（153.7％），Mn ⁺⁺（106.3％），Ba ⁺⁺（181.9％），Ni ⁺⁺（107.2％），而被Fe ⁺⁺（15.8％），β-巯基乙醇（46.8％）抑制），EDTA（54.5％）。焓，自由能和激活的熵分别计算为38.526 kJmol ^-1，44.187 kJmol ^-1，和-17.518的Jmol ^-1 ķ ^-1分别。另外，发现ΔGE _-S和ΔGE _-T为-10.75kJmol ^-1和-45.92 kJmol ^-1。较低的ΔS，ΔG _E-S和ΔG _E-T值表示酶催化的反应快速发生，并且酶以稳定状态存在。纤维二糖是水解的主要最终产物。GluCB31的这些属性证明了催化活性的多样性，并在各种生物技术过程中发挥作用，因此值得作为生物工业剂开发。