Diaporthe (syn. Phomopsis) species have often been reported as plant pathogens, endophytes or saprobes, commonly isolated from a wide range of hosts. In Brazil, in orchards located in Monte Azul, Minas Gerais, flowers and unripe fruits of Passiflora edulis were found with very severe and typical Phomopsis rot symptoms. The aim of this study was to prove the pathogenicity of the fungus isolated from symptomatic flowers and fruits, and to identify the pathogen’s species. The isolate was identified on the basis of DNA sequence analyses based on the nuclear ribosomal internal transcribed spacer region and part of the translation elongation factor-1α. On culture media the colonies were sterile and pycnidia appear only on the surface of fruits. The first symptoms of the disease appeared 5 days after inoculation in flowers and after 11 days in ripe and unripe fruits inoculated at wounded sites. The pathogen was identified as Diaporthe infecunda and was re-isolated from those lesions, confirming Koch’s postulates. D. infecunda has been reported only as plant endophytic fungus and to our knowledge, this is the first confirmed report as a plant pathogenic fungus, causing rot in flowers and, in ripe and unripe fruits of P. edulis in the world.

中文翻译：

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Diaporthe infecunda 对巴西西番莲果实和花朵造成的拟茎点腐病

Diaporthe (syn. Phomopsis) 物种经常被报道为植物病原体、内生菌或腐生菌，通常从广泛的宿主中分离出来。在巴西，在位于米纳斯吉拉斯州 Monte Azul 的果园中，发现西番莲的花朵和未成熟果实具有非常严重和典型的拟茎点霉腐烂症状。本研究的目的是证明从有症状的花和果实中分离出的真菌的致病性，并确定病原体的种类。根据核糖体内部转录间隔区和部分翻译延伸因子-1α，在 DNA 序列分析的基础上鉴定了分离株。在培养基上，菌落是无菌的，分生孢子仅出现在果实表面。该病的最初症状出现在花接种后 5 天，以及在受伤部位接种成熟和未成熟果实 11 天后出现。病原体被鉴定为不育病菌，并从这些病变中重新分离，证实了科赫的假设。D. infecunda 仅被报告为植物内生真菌，据我们所知，这是世界上第一个确认报告为植物病原真菌，导致花和成熟和未成熟的 P. edulis 果实腐烂。