Bacterial adhesion is considered as an important property that enables probiotic bacteria to colonise the gastrointestinal tract and confer a health benefit to the host. In the present study, a shrimp gut isolate Bacillus subtilis subsp. inaquosorum V₁, an efficient producer of bacteriocin was used. Autoaggregation, co-aggregation and cell surface hydrophobicity assays were performed to confirm the aggregating phenotype of the strain. The results of the assays showed that the strain belonged to a strong aggregating phenotype, autoaggregation (45.7%) and co-aggregation with the pathogens like E. coli (33.17%) and S. aureus (35.7%). It also aggregates with the hydrocarbons such as xylene (44.8%) and toluene (52.67%). The effect of LiCl₂ on bacterial cells indicated the role of S-layer proteins on the bacterial adhesion in which LiCl₂-treated cells showed minimal adhesion (16.47%), when compared with untreated cells (45.3%). The candidate strain was supplemented in a commercial fish feed and fed to the ornamental fish Carassius auratus. After 30 days, the experimental group of fishes showed an increase in weight gain (34.56%) with the SGR of 0.99% than the control group. The enzymes to toxify the respiratory oxidative stress, namely SOD, catalase and the digestive enzyme activity were found to be more in the experimental fishes. Similarly, the total protein content of the experimental fishes showed a marked increase than the control diet–fed fish. The above results inferred that the experimental strain has the better adhesive property and can be used as an effective probiotic to improve the production in aquaculture.

细菌粘附被认为是使益生菌能够在胃肠道定居并为宿主带来健康益处的重要特性。在本研究中，虾肠分离枯草芽孢杆菌亚种。在aaquosorum V _1中，使用了高效的细菌素生产商。进行自动聚集，共聚集和细胞表面疏水性测定以确认菌株的聚集表型。分析结果表明，该菌株属于强聚集表型，自聚集（45.7％）并与大肠杆菌（33.17％）和金黄色葡萄球菌等病原体共聚集。（35.7％）。它还与二甲苯（44.8％）和甲苯（52.67％）等烃类聚集。LiCl ₂对细菌细胞的作用表明了S层蛋白对细菌粘附的作用，其中与未处理的细胞（45.3％）相比，LiCl ₂处理的细胞显示出最小的粘附（16.47％）。将候选菌株添加到市售鱼饲料中，并喂入观赏鱼Car鱼。30天后，鱼的实验组显示体重增加（34.56％），SGR为0.99％。在实验鱼类中发现能毒性化呼吸氧化应激的酶，即SOD，过氧化氢酶和消化酶活性更高。同样，实验鱼类的总蛋白质含量也比对照饮食喂养的鱼类明显增加。由以上结果可知，该实验菌株具有更好的粘合性能，可以作为提高水产养殖产量的有效益生菌。