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Production of concentrated inocula from the microalgae Nannochloropsis oculata
Aquaculture International ( IF 2.9 ) Pub Date : 2020-04-10 , DOI: 10.1007/s10499-020-00546-9
Rafael Sales , Lília Pereira de Souza-Santos

Aquaculture farms, such as the ones that produce mollusks, marine fish, and shrimp, require a constant supply of microalgae biomass as feed. Microalgae cultures are subject to failures in continuity; therefore, concentrated inocula could be used during episodes of crashes to reestablish the production of microalgae biomass. For this reason, the present work evaluated a concentrated inocula of the microalgae Nannochloropsis oculata harvested by flocculation and stored in a refrigerator with additives (vitamin C or citric acid) and without additives. Sensorial analysis (color and odor), protein content, cell integrity rate, cell resuspension rate, and culture growth parameters (maximum cell density and growth rate) were estimated for 15 weeks. The concentrates with citric acid presented an alteration in the color aspect at the 11th week of storage. No differences were seen in the protein content and cell integrity rates either between storage methods or storage weeks. In sum, the use of additives did not significantly influence the growth performance of any of the treatments, since their cells continued to reproduce throughout the entire trial period. In addition, the additives enhanced the cell resuspension rate of the concentrates. For that reason, the concentrated inoculum with vitamin C was selected as the best treatment.



中文翻译:

从微藻Nannochloropsis oculata生产浓缩接种物

水产养殖场,例如生产软体动物,海鱼和虾的场,需要不断供应微藻生物质作为饲料。微藻培养会遭受连续性的破坏;因此,在事故期间可以使用浓缩接种物来重新建立微藻生物质的生产。因此,本工作评估了微藻Nannochloropsis oculata的浓缩接种物通过絮凝收获,并与添加剂(维生素C或柠檬酸)一起储存在冰箱中,不添加任何添加剂。估计15周的感官分析(颜色和气味),蛋白质含量,细胞完整性率,细胞重悬率和培养物生长参数(最大细胞密度和生长率)。用柠檬酸浓缩物呈现在彩色方面的改变在11储存一周。在储存方法或储存周之间,蛋白质含量和细胞完整性速率均未见差异。总而言之,添加剂的使用不会显着影响任何处理的生长性能,因为它们的细胞在整个试验期间都持续繁殖。另外,添加剂提高了浓缩物的细胞重悬浮速率。因此,选择含维生素C的浓缩接种物为最佳治疗方法。

更新日期:2020-04-18
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