The increase of ammonia nitrogen, nitrite, nitrate, urea, and other dissolved nitrogen in prawn pond often causes eutrophication of water body and prawn poisoning, which brings great harm to aquaculture. Microalgae are the main biological factors in prawn pond, which can effectively utilize dissolved nitrogen. The goal of this study was to evaluate the effects of the forms and concentrations of various dissolved nitrogen on the uptake rate (ρ) by Oocystis borgei, and establish the relationship model between ammonia nitrogen uptake rate and key environmental factors using ¹⁵N isotope labeling technique. Temperature (A), light intensity (B), salinity (C), pH (D), and algal concentration (E) were the factors used to construct an empirical model. Study results showed that nitrogen concentrations had a significant effect on the uptake rates of ammonium, nitrate, nitrite, and urea (P < 0.05). Under various salinity and temperature conditions, the relative preference index (RPI) of ammonium was greater than that of nitrite, urea, and nitrate; Ammonium was the preferred nitrogen source for O. borgei. The optimal combination of environmental conditions for ρ(NH₄⁺-N) was temperature of 20 °C, pH of 7.5, light intensity of 81 μmol m⁻² s⁻¹, salinity of 15‰, and algal concentration of 5.5 × 10⁸ cell L⁻¹. The model equation wasρ = 0.0017 × (A^0.180B^0.252C^0.748D^0.587E) + 0.0512, with the coefficient of determination (R²) of 0.89. No significant difference was observed between the model-predicted values and the measured values (F = 0.789, P > 0.05), which demonstrated the high fitting degree of simulation equation. This study provides valuable insight into the removal of dissolved nitrogen in a controllable prawn farming mode such as high-elevation ponds by O. borgei.

虾塘中氨氮，亚硝酸盐，硝酸盐，尿素及其他溶解氮的增加，经常引起水体富营养化和虾中毒，给水产养殖业带来极大危害。微藻是虾塘中的主要生物因子，可以有效利用溶解氮。本研究的目的是评估各种溶解氮的形态和浓度对博氏oocystis borgei吸收率（ρ）的影响，并利用¹⁵ N同位素标记技术建立氨氮吸收率与关键环境因子的关系模型。。温度（A），光强度（B），盐度（C），pH（D）和藻类浓度（E）是用于建立经验模型的因素。研究结果表明，氮浓度对铵，硝酸盐，亚硝酸盐和尿素的吸收速率有显着影响（P <  0.05）。在不同盐度和温度条件下，铵的相对优先指数（RPI）大于亚硝酸盐，尿素和硝酸盐。铵是博尔盖球菌的优选氮源。ρ（NH ₄ ⁺ -N）的环境条件的最佳组合是温度为20°C，pH为7.5，光强度为81μmolm ^-2  s ^-1，盐度为15‰，藻类浓度为5.5×10 ⁸ 单元格L ^-1。模型方程为ρ  = 0.0017×（甲^0.180乙^0.252 Ç ^0.748 d ^0.587 ë）+ 0.0512，与决定系数（- [R ²的0.89）。模型预测值与实测值之间无显着差异（F =  0.789，P  > 0.05），表明模拟方程的拟合度较高。这项研究提供了有价值的见解，可用于以可控制的虾类养殖模式（如博格氏菌（O. borgei）的高海拔池塘）去除溶解氮的方法。