The products released by the corpus allatum (CA), an endocrine gland producing juvenile hormone (JH), were analyzed in three species of stink bugs. Liquid chromatography–mass spectrometry (LC–MS) analysis of the CA products from female adults of Halyomorpha halys (Stål), Nezara viridula (Linnaeus), and Nezara antennata Scott (Hemiptera: Pentatomidae) revealed that the CA biosynthesized JH III skipped bisepoxyside (JHSB3) in all three species. In H. halys, in addition to JHSB3, the CA also produced its stereoisomer, 10S-JHSB3. A bioassay focusing on the number of antennal segments was adopted to examine biological activity of these products. When last instar nymphs were treated with either of the two JHs, they emerged as adults bearing nymphal type antennae with four segments in a dose-dependent fashion, indicating that both JHSB3 and 10S-JHSB3 had the JH activity in H. halys. Therefore, for the first time, 10S-JHSB3 in H. halys was found to be a naturally occurring JH molecule with 10S-configuration.

中文翻译：

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具有 10S 构型的 JHSB3 立体异构体 10S-JHSB3，由褐蠹蝽的 corpus allatum 生物合成，Halyomorpha halys（半翅目：五足科）

在三种臭虫中分析了由产生保幼激素 (JH) 的内分泌腺体 (CA) 释放的产物。对 Halyomorpha halys (Stål)、Nezara viridula (Linnaeus) 和 Nezara 触角 Scott (Hemiptera: Pentatomidae) 雌性成虫的 CA 产物进行液相色谱-质谱 (LC-MS) 分析表明，CA 生物合成的 JH III 跳过了双环氧苷（ JHSB3) 在所有三个物种中。在 H. halys 中，除了 JHSB3 外，CA 还产生了其立体异构体 10S-JHSB3。采用侧重于触角节数的生物测定来检查这些产品的生物活性。当最后一龄若虫用两种 JH 中的任何一种进行治疗时，它们以剂量依赖性方式出现带有四节的若虫型触角的成虫，表明 JHSB3 和 10S-JHSB3 在 H. halys 中均具有 JH 活性。因此，首次发现 H. halys 中的 10S-JHSB3 是一种天然存在的具有 10S 构型的 JH 分子。