An 8-y-old castrated male, outdoor European shorthair cat was presented with a history of hindlimb weakness and paralysis. Disease progression was continuous from the onset; deep algesia disappeared at the final stage. Radiography of the vertebral column was unremarkable; along with patient history and physical examination results, magnetic resonance imaging suggested inflammatory lesions in the spinal cord, although neoplasia could not be ruled out. Feline leukemia virus (FeLV) positivity was confirmed by a serum ELISA prior to euthanasia. Upon postmortem examination, hemorrhages were present in the spinal cord at the level of vertebrae T7-8. Histologic and immunohistochemical analysis revealed primary diffuse large B-cell lymphoma of the spinal cord with multifocal myelomalacia and hemorrhages. To determine the presence of a pathogen within the lesion, we developed a novel in situ hybridization protocol for FeLV (RNAscope). The reaction revealed large amounts of FeLV viral RNA in the tumor cells.

中文翻译：

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在原发性神经B细胞淋巴瘤中猫白血病病毒的原位杂交。

出现了8岁的cast割的雄性户外欧洲短毛猫，有后肢无力和麻痹的病史。从发病开始，疾病进展是连续的；在最后阶段深层痛觉消失。脊柱X线摄片不明显。连同患者病史和体格检查结果一起，磁共振成像提示脊髓炎性病变，尽管不能排除肿瘤。在安乐死之前通过血清ELISA确认猫白血病病毒（FeLV）阳性。验尸后，脊髓中的出血水平为椎骨T7-8。组织学和免疫组织化学分析显示，原发性脊髓弥漫性大B细胞淋巴瘤伴多灶性脊髓软化和出血。为了确定病灶内病原体的存在，我们开发了一种针对FeLV（RNAscope）的新型原位杂交方案。该反应显示肿瘤细胞中有大量FeLV病毒RNA。