We characterized the antibody response to decorin-binding protein A (DbpA) or DbpB from immune serum samples collected from 27 dogs infected with Borrelia burgdorferi by Ixodes scapularis ticks. Immunoglobulin M (IgM) antibodies to DbpA or DbpB were rarely detected, but high levels of IgG antibodies to DbpA were detected in 16 of 27 of the immune sera collected 1 mo after infection, 20 of 25 of the sera collected after 2 mo, and each of the 23, 17, or 11 serum samples evaluated after 3, 4, or 5 mo, respectively. In addition, IgG antibodies to DbpB were detected in 22 of 27 (p = 0.005) tested dogs after 1 mo, and the frequency of detecting the antibodies thereafter closely mimicked the antibody responses to DbpA. Moreover, antibodies to DbpA or DbpB were not produced by dogs vaccinated with a whole-cell B. burgdorferi bacterin; removing the antibodies to DbpA by adsorption to recombinant DbpA (rDbpA) did not affect the reactivity detected by a rDbpB ELISA. Therefore, the findings from our preliminary study showed that antigenically distinct antibodies to DbpA or DbpB are produced reliably during canine infection with B. burgdorferi, and the response is not confounded by vaccination with a Lyme disease bacterin. Larger studies are warranted to more critically evaluate whether detecting the antibody responses can improve serodiagnostic confirmation of canine Lyme disease.

中文翻译：

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用壁虱攻击法检测犬伯氏疏螺旋体感染狗后，对核心蛋白聚糖结合蛋白A（DbpA）和DbpB的抗体检测。

我们表征了从蝶粉虱感染了27只感染了伯氏疏螺旋体的狗的免疫血清样品中对decorin结合蛋白A（DbpA）或DbpB的抗体应答。很少检测到针对DbpA或DbpB的免疫球蛋白M（IgM）抗体，但是在感染后1个月收集的27份免疫血清中有16份，在2个月后收集的25份血清中有20份检测到高水平的DbpA IgG抗体。在分别于3、4或5 mo后评估的23、17或11个血清样本中的每一个。此外，在1个月后的27只（p = 0.005）测试的狗中，有22只检测到针对DbpB的IgG抗体，此后检测抗体的频率非常类似于抗体对DbpA的反应。而且，接种全细胞伯氏疏螺旋体细菌的狗没有产生针对DbpA或DbpB的抗体。通过吸附到重组DbpA（rDbpA）中除去针对DbpA的抗体，不会影响rDbpB ELISA检测到的反应性。因此，我们初步研究的结果表明，在犬博德多福氏杆菌感染过程中，可靠地产生了针对DbpA或DbpB的抗原独特抗体，并且接种莱姆病细菌不会引起反应混淆。必须进行更大的研究，才能更严格地评估检测抗体反应是否可以改善犬莱姆病的血清诊断。并且接种莱姆病细菌菌苗不会混淆反应。必须进行更大的研究，才能更严格地评估检测抗体反应是否可以改善犬莱姆病的血清诊断。并且接种莱姆病细菌菌苗不会混淆反应。必须进行更大的研究，才能更严格地评估检测抗体反应是否可以改善犬莱姆病的血清诊断。