Immune responsive protein 30 (IRP30) is a Hymenoptera-specific protein first identified from honey bee hemolymph in response to bacterial infection. However, its function remains elusive. Here, we cloned the full-length IRP30 gene and clarified its expression pattern in the bumble bee Bombus lantschouensis (Vogt). The full-length IRP30 gene measures 1443 bp and contains two exons and one intron. The length of the cDNA is 1082 bp, including a 36-bp 5′-UTR and a 218-bp 3′-UTR, and it encodes a putative protein of 275 amino acids. As expected, the sequence of the B. lantschouensis IRP30 protein was clustered with the bumble bee group, which appeared as a single clade next to honey bees. The family shared similar conserved protein domains. Moreover, bumble bee IRP30 belongs to a recently diverged clade that has four leucine-rich repeat (LRR) conserved domains. IRP30 is highly expressed in the worker caste, during pupal developmental stages, and in the head and thorax tissues. Interestingly, its expression increases 20- to 90-fold when female bumble bees (B. lantschouensis) and honey bees (Apis mellifera L.) begin laying eggs. Overall, based on the expression of IRP30 during development and egg laying in female bumble bees, this protein not only responds to immune challenge but also may play an important role in metamorphosis and reproduction.

中文翻译：

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大黄蜂Lantchouensis（膜翅目：Apididae）的免疫反应蛋白IRP30的新特征。

免疫应答蛋白30（IRP30）是膜翅目特异性蛋白，最早是从蜜蜂的血淋巴中发现以响应细菌感染。但是，其功能仍然难以捉摸。在这里，我们克隆了全长IRP30基因，并阐明了其在大黄蜂Bosbus lantschouensis（Vogt）中的表达方式。全长IRP30基因的长度为1443 bp，包含两个外显子和一个内含子。cDNA的长度为1082 bp，包括36 bp的5'-UTR和218-bp的3'-UTR，编码275个氨基酸的推测蛋白。不出所料，兰氏芽孢杆菌的序列IRP30蛋白与大黄蜂群聚集在一起，在蜜蜂旁边显示为单个分支。该家族共有相似的保守蛋白结构域。此外，大黄蜂IRP30属于最近分化的进化枝，具有四个富含亮氨酸的重复序列（LRR）保守结构域。IRP30在工人种姓，p发育阶段以及头和胸组织中高表达。有趣的是，当雌性大黄蜂（B. lantschouensis）和蜜蜂（Apis mellifera L.）时，其表达增加20到90倍。）开始产卵。总的来说，基于IRP30在雌性大黄蜂发育和产卵过程中的表达，该蛋白不仅对免疫挑战做出反应，而且可能在变态和繁殖中发挥重要作用。