The bacterial community living in the insect gut may play an important role in nutrition, immunity and protection, detoxification of toxins, and inter- and intra-specific communication. Rice leaffolder Cnaphalocrocis medinalis (Guenée) (Lepidoptera: Crambidae) is a notorious pest in rice, and the diversity of the gut bacteria of C. medinalis across life stages are not well understood. Here, the diversity and abundance of the gut bacterial community in C. medinalis through life stages were investigated using Illumina Miseq technology. A total of 22 bacterial phyla, 42 classes, 100 orders, 179 families, 350 genera and 395 species were identified across the different life stages of C. medinalis. Proteobacteria and Firmicutes phyla were the dominant bacterial taxa. Members of the genera Enterococcus, unclassified Enterobacteriaceae, Wolbachia, Acinetobacter, Stenotrophomonas, Microbacterium, Bacillus, Corynebacterium, Lampropedia, and Sphingobacterium were found at all life stages. Enterococcus and unclassified Enterobacteriaceae occupied higher relative abundance among bacteria community in the 2nd to 5th instar larvae, pupae and adults. The structure of bacterial community differed across the life stages of C. medinalis. Our findings will enrich the understanding of gut bacteria in C. medinalis, and will provide foundation and assistance for the development of novel pest management strategies through utilization of microbiota.

生活在昆虫肠道中的细菌群落可能在营养，免疫和保护，毒素排毒以及种间和种内交流中起重要作用。稻纵卷叶C Cnaphalocrocis medinalis（Guenée）（鳞翅目：Crambidae）是稻米中的臭名昭著的害虫，人们对整个生命周期中me稻C. medinalis肠道细菌的多样性知之甚少。在这里，在肠道细菌群落的多样性和丰富稻纵卷叶螟的人生阶段都利用Illumina Miseq技术研究。在整个生命周期中，共鉴定出22种细菌门，42类，100个目，179个科，350属和395种。。变形杆菌和菌门是主要的细菌类群。该属的成员肠球菌属，肠杆菌科机密，沃尔巴克氏体，不动杆菌，嗜，微杆菌，芽孢杆菌，棒状杆菌，Lampropedia，和鞘氨醇在所有生命阶段被发现。在第二至第五龄幼虫，p和成虫中，肠球菌和未分类的肠杆菌科细菌群落中相对丰度较高。在整个C. medinalis生命周期中，细菌群落的结构各不相同。我们的发现将丰富对中脉肠线虫肠道细菌的了解，并将为通过利用微生物群开发新的病虫害防治策略提供基础和帮助。