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Wax glands ultrastructure and chemical composition of wax of giant mealybug Drosicha stebbingii (Green) (Hemiptera: Monophlebidae)
Journal of Asia-Pacific Entomology ( IF 1.1 ) Pub Date : 2020-03-17 , DOI: 10.1016/j.aspen.2020.03.009
Ayashaa Ahmad , Aditi Kundu , Debjani Dey

Droschia stebbingii (Green) belonging to the family Monophlebidae (Hemiptera: Coccoidea) is a serious, phytophagous and dimorphic pest. D. stebbingii and D. mangiferae (Green) with sixty-two common hosts and minor morphological variations pose problems towards their taxonomic identification. Light and scanning electron microscopy were used to outline the ultrastructural details of cuticular wax producing structures of D. stebbingii. Three types of wax-pores along with tubular duct were observed on the head, thorax and abdomen. Quinquelocular and multilocular disc-pores exhibited variations in the central loculus with single and double opening. Short curly wax strands were secreted from the outer loculi whereas central loculus secreted long tubular strands. Additionally, bilocular pores secreting curly wax were observed having two large loculi separated by a septum. GC–MS analysis of cuticular wax indicated the presence of hydrocarbons and wax esters forming majority of the covering along with long chain alcohols, ketones and acids. Variations in the percentage of hydrocarbon and wax esters content were observed for two populations of D. stebbingii collected from different hosts. Hydrocarbons were abundant in populations collected on Pongamia pinnata (L.) Pierre whereas long chain esters dominated the wax profile for the population collected from Tectona grandis L.f. Compositional variations in the cuticular wax of D. stebbingii on different host along with knowledge on the ultrastructural wax pores and waxy secretion would provide an insight to the existing knowledge of this scale insect.



中文翻译:

巨型粉虱Drosicha stebbingii(绿色)的蜡腺超微结构和蜡的化学组成(半翅目:单生科)

属于单毛纲科(半翅目:球虫科)的果蝇Droschia stebbingii)(绿色)是一种严重的,植物性和双态性害虫。D. stebbingiid六十二个常见寄主和微小形态变异的芒果(绿色)对其分类学识别提出了问题。用光学和扫描电子显微镜勾勒出D. stebbingii表皮蜡产生结构的超微结构细节。在头部,胸部和腹部观察到三种类型的蜡孔以及管状导管。五眼和多眼的盘孔在中央眼中显示出单孔和双孔的变化。短的蜡状蜡束从外耳房分泌,而中央的叶肉分泌长的肾小管股。另外,观察到分泌卷曲蜡的双眼毛孔具有由隔膜隔开的两个大的室。对表皮蜡的GC-MS分析表明,与长链醇,酮和酸一起存在的碳氢化合物和蜡酯构成了覆盖物的大部分。观察到从不同寄主收集的两个D. stebbingii种群的碳氢化合物和蜡酯含量的百分比变化。碳氢化合物的收集人口众多Pongamia pinnata(L.)Pierre,而长链酯则主导了从Tectona grandis Lf收集的种群的蜡像分布。不同宿主上D. stebbingii的表皮蜡的组成变化以及对超微结构蜡孔和蜡质分泌物的了解将提供洞察这种鳞片昆虫的现有知识。

更新日期:2020-03-17
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