The morphological characters of honeybees have an important role for discriminating honeybee subspecies. In the present research, Iranian populations of honeybee (Apis mellifera) were collected from 19 areas in Iran. The samples were collected from stationary beekeeping sites. Moreover, pictures of honeybee forewings held in the Bee Data Bank in Oberursel were compared with Iranian honeybee populations. 19 morphological characters were measured for each forewing of worker honeybee to evaluate differentiation of Iranian honeybee populations from the commercial honeybee subspecies A. m. mellifera, A. m. carnica, A. m. caucasica and A. m. ligustica. Additionally, part of the tRNA^leu gene, an intergenic region and part of COII was used to confirm differentiation of the commercial subspecies from Iranian honeybee populations. Results of the cluster analyses showed that 19 morphological characters of forewings differentiated Iranian populations from the commercial subspecies. Moreover, the phylogenetic tree of part of the tRNA^leu gene, an intergenic region and part of COII differentiated the commercial subspecies from Iranian honeybee populations. Results of the discriminant function analyses (DFA) indicated that the references samples of A. m. meda overlapped with Iranian populations.

蜜蜂的形态特征对于区分蜜蜂亚种具有重要作用。在本研究中，从伊朗的19个地区收集了伊朗蜜蜂（蜜蜂）。样品是从固定的养蜂场收集的。此外，将Oberursel的Bee数据库中保存的蜜蜂前翅照片与伊朗蜜蜂种群进行了比较。每次测量工蜂的前翅测量19个形态特征，以评估伊朗蜜蜂种群与商业蜜蜂亚种A.m.的区别。西方蜜蜂，A.米。carnica，A.米。高加索和米 女贞。此外，部分tRNA^亮氨酸 该基因，一个基因间区域和部分COII被用于确认伊朗蜜蜂种群与商业亚种的区别。聚类分析结果表明，前翅的19个形态特征将伊朗种群与商业亚种区分开来。此外，部分tRNA ^leu 基因，一个基因间区域和一部分COII的系统树将商业亚种与伊朗蜜蜂种群区分开。判别函数分析（DFA）的结果表明A.m的参考样品。meda与伊朗人口重叠。