Acridids are highly abundant living-organism/major-component in agricultural field, globally. It may act as a dependable bio-indicator species in response to environmental-stress. Antioxidants protect insects by scavenging free radicals. Here, we investigated the dose dependant azadirachtin (C₃₅H₄₄O₁₆ AZT) toxicity on oxidative-biomarker; Alkaline-phosphatase (ALP), malondialdehyde (MDA), non-protein-soluble-thiol (NPSH) and antioxidants like superoxide-dismutase (SOD), catalase (CAT), glutathione-peroxidase (GPx) and amylase of gonads from both sexes and juvenile tissues of common grasshopper, Spathosternum prasiniferum prasiniferum (Walker, 1871). The newly hatched adults (female: male = 1:1) are exposed to 1 to 20 ppm AZT for 48 h and have compared to control and 6 h incubation with same concentration of azadirachtin for in vitro experiments. Both in vivo and in vitro experiment demonstrated significant influences on oxidative biomarkers with increasing antioxidant enzymatic activities in either sex. The male gonads represents decreasing antioxidant enzyme activities compared to female gonads. Lesser protection by CAT and SOD are noticed in male than female in response to AZT exposure. This experiment suggests that azadirachtin increased the major biomarkers with decreasing antioxidant enzyme activities resulting in more free-radicals related threat in adult male gonads. Variable dose responses were noticed on ALP, AchE and MDA in either gender suggesting multiphasic action of the pesticides. Higher mortality rate is noticed in male with lower nymphal life span. Moreover, nymph II^nd is more susceptible than nymph IV^th in vitro intoxication of azadirachtin. Possible life threat of vast representatives of agricultural ecosystem by pesticide should be avoided to maintain different bio-geo cycle and eco-sustainability.

rid虫类是全球农业领域中高度丰富的生物/主要成分。它可以作为应对环境压力的可靠生物指示剂。抗氧化剂通过清除自由基来保护昆虫。在这里，我们研究了剂量依赖性印za素（C ₃₅ H ₄₄ O ₁₆ AZT）对氧化生物标志物的毒性。碱性磷酸酶（ALP），丙二醛（MDA），非蛋白质可溶性硫醇（NPSH）和抗氧化剂，如两性腺中性腺的超氧化物歧化酶（SOD），过氧化氢酶（CAT），谷胱甘肽过氧化物酶（GPx）和淀粉酶蚱grass和白色蚱ju的幼体和幼体组织prasiniferum（沃克，1871年）。新孵化的成年人（女性：男性= 1：1）暴露于1至20 ppm的AZT 48小时，并与对照组和相同浓度的印za素进行6小时孵育进行体外实验。体内和体外实验均表明，随着性别的增长，抗氧化酶活性的增加对氧化生物标志物的影响也很大。与雌性性腺相比，雄性腺代表减少的抗氧化酶活性。对于AZT暴露，男性和女性对CAT和SOD的保护作用较小。该实验表明印za素增加了主要的生物标志物，同时抗氧化酶的活性降低，导致成年男性性腺中与自由基相关的威胁更大。在ALP上注意到剂量反应不同，两种性别的AchE和MDA均表明该农药具有多相作用。若虫寿命越短，男性死亡率越高。此外，若虫II^第二比若虫IV更易感^个在印楝素的体外中毒。应避免农药对农业生态系统的巨大代表可能造成的生命威胁，以维持不同的生物地理周期和生态可持续性。