Abstract This study aimed to investigate the effect of a novel oral somatostatin-14 (SS-14) DNA vaccine fused to tissue plasminogen activator (tPA) signal peptide and CpG adjuvant on goat lactation and milk composition. Ten female goats with two newborn kids each were randomly divided into the immunized group and negative control group; The novel oral somatostatin (SS) DNA vaccine (fused to tPA signal peptide and CpG adjuvant) and empty vector bacteria were orally administered to these groups, respectively, at weeks 0, 3, and 6 of the study period. Blood samples were collected before primary immunization and at weeks 6 and 9 after primary immunization. The specific anti-SS antibody titers in the immunized group were significantly higher than that in the negative control group (P

中文翻译：

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与 tPA 信号肽和 CpG 佐剂融合的新型生长抑素 14 DNA 疫苗对山羊泌乳和牛奶成分的影响

摘要 本研究旨在研究融合组织纤溶酶原激活剂 (tPA) 信号肽和 CpG 佐剂的新型口服生长抑素 14 (SS-14) DNA 疫苗对山羊泌乳和牛奶成分的影响。10只母山羊，每只2只，随机分为免疫组和阴性对照组；在研究期间的第 0、3 和 6 周，分别向这些组口服新型口服生长抑素 (SS) DNA 疫苗（与 tPA 信号肽和 CpG 佐剂融合）和空载体细菌。在初次免疫前和初次免疫后第 6 周和第 9 周收集血样。免疫组特异性抗SS抗体滴度显着高于阴性对照组（P