Abstract Dipteryx alata Vog. is a forest species native to the Brazilian Cerrado with great economic potential, but information on seed behavior during germination and accelerated aging is lacking. Thus, the objective of this study was to evaluate the quality of D. alata seeds during germination and to measure the possible damage caused by aging. The physiological performance of D. alata seeds was investigated via two experiments: (1) imbibition curve, where the seeds were stored in a Biochemical Oxygen Demand chamber (B.O.D) at 30 °C and 60% of relative humidity (RH), for eight days, and (2) accelerated aging, where the seeds were stored in a B.O.D at 42 °C and 60% of RH, for 96 h. In the experiment 1, α- and β-amylase activity and histochemical changes were determined in endosperms and embryos collected at 0, 6, 12, 24, 36, 48, 96, 144 and 196 h after imbibition. In the experiment 2, respiratory activity, electrical conductivity, and catalase, superoxide dismutase and peroxidase activity, as well as protein content and vigor tests, were determined in embryos collected at 0, 48, 72 and 96 h after accelerated aging treatment. The results indicated that α- and β-amylase synthesis was activated after 48 h of imbibition, culminating in starch degradation. Concomitant with starch degradation, lipids and proteins were consumed throughout the germination process. Accelerated aging promoted an increase in respiration, electrical conductivity and peroxidase activity as well as a reduction in superoxide dismutase and catalase activity. And, although the germination rate of D. alata seeds was not compromised by accelerated aging, a decrease in the germination speed index was observed over time. Thus, together, these biochemical and physiological changes can be used as markers of D. alata seed deterioration induced by high temperatures and humidity.

中文翻译：

---

Dipteryx alata Vog 的生化和生理变化。种子发芽和加速老化

摘要 Dipteryx alata Vog。是一种原产于巴西塞拉多的森林物种，具有巨大的经济潜力，但缺乏关于发芽和加速老化过程中种子行为的信息。因此，本研究的目的是评估发芽期间 D. alata 种子的质量，并测量老化可能造成的损害。通过两个实验研究了 D. alata 种子的生理性能：（1）吸胀曲线，其中将种子储存在 30 °C 和 60% 相对湿度（RH）的生化需氧室（BOD）中，8天，和 (2) 加速老化，其中种子在 42 °C 和 60% RH 的 BOD 中储存 96 小时。在实验 1 中，在 0、6、12、24、36、48、96、吸入后 144 和 196 小时。在实验2中，在加速老化处理后0、48、72和96小时收集的胚胎中测定呼吸活性、电导率以及过氧化氢酶、超氧化物歧化酶和过氧化物酶活性，以及​​蛋白质含量和活力测试。结果表明 α- 和 β- 淀粉酶合成在吸收 48 小时后被激活，最终导致淀粉降解。伴随淀粉降解，在整个发芽过程中消耗脂质和蛋白质。加速老化促进呼吸、电导率和过氧化物酶活性的增加以及超氧化物歧化酶和过氧化氢酶活性的降低。而且，虽然 D. alata 种子的发芽率没有因加速老化而受到影响，随着时间的推移，观察到发芽速度指数下降。因此，这些生化和生理变化可以一起用作高温和高湿引起的 D. alata 种子退化的标志。